Palma RA, Monroy NA, Castrillón RL, Castañeda SJI, Padilla DMC

Language: Spanish
References: 24
Page: 5-9
PDF size: 305.35 Kb.

ABSTRACT

Background: Mycetoma is a chronic infection of the skin and underlying tissues with a tendency to affect bones. It is characterized by increased volume and fistulas. When the causative agent is an actinomycete, it is called an actinomycetoma, where Actinomadura madurae is the cause of 7.9% of cases in Mexico and predominates in the female sex. Toll-like receptors (2 and 4) in skin sections of five patients with this infection were studied since the keratinocytes present play an important role in the innate immunity response of this tissue, secreting proinflammatory cytokines, antimicrobial peptides, inducible enzymes, and adhesion molecules. Material and methods: Five blocks of paraffin were used with tissue from patients diagnosed with actinomycetoma due to Actinomadura madurae and one from normal human skin (control), treated at the Dermatological Center «Dr. Ladislao de la Pascua». Three cuts were made per sample, one to perform hematoxylin and eosin (H&E) staining, and two to perform immunohistochemical techniques for TLR2 and TLR4, using the human IgG anti-TLR2 or IgG anti-TLR4 antibodies made in mouse, and the interaction was revealed with a commercial kit (Cell and Tissue Staining Kit, mouse Kit, HRP-AEC System by R&D Minneapolis, MN, USA Cat No. CTS003). Results: When performing the immunohistochemical technique on the sections with actinomycetoma due to Actinomadura madurae to demonstrate the presence of TLR2 and TLR4, red coloration is observed in the keratinocyte area, although with greater intensity for TLR4 than for TLR2. Conclusion: The presence of TLR4 is higher compared to TLR2 in skin keratinocytes with Actinomadura madurae actinomycetoma, in the five patients studied by the in situ hybridization technique.

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