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ISSN 2683-1678 (Print)
Órgano Oficial de la Sociedad
Latinoamericana de lnfectología Pediátrica.
Órgano de la Asociación Mexicana de
Infectología Pediátrica, A.C.
Órgano difusor de la Sociedad Española
de lnfectología

2020, Number 3

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Rev Latin Infect Pediatr 2020; 33 (3)

Isolation of intestinal spirochetes Brachyspira spp., (enteropathogenic to swine and other domestic animals) from stool samples submitted to public hospital

Corona BE, Salvador TDI, Ortiz MMG, Barrios GH, Vázquez VJ, VanKley A

Language: Spanish
References: 19
Page: 125-130
PDF size: 214.43 Kb.


ABSTRACT

Brachyspira spp. are intestinal spirochetes that are important in veterinary medicine, the pig is the host for which more studies have been published. The intestinal spirochetes are responsible intestinal infections that cause muco-hemorragic diarrhea, colitis and intestinal disorders in various hosts including humans. Two species of Brachyspira affect people, Brachyspira pilosicoli (also affects the pig and other domestic animals) and Brachyspira aalborgi. The aim of this work was to demonstrate evidence of the genus Brachyspira in fecal samples sent to parasitology laboratory of two public hospitals in Ciudad Victoria, Tamaulipas, Mexico. Fecal samples were obtained from Hospital Civil de Tamaulipas (Civil Hospital of Tamaulipas, n = 160) and from Hospital Infantil de Tamaulipas (Children Hospital of Tamaulipas, n = 65). Samples were seeded onto selective bacteriological medium and incubated in anaerobic jar at 42 oC for 7 days for isolation of Brachyspira. Out of 225 samples, 5 were positive to bacteriological isolation, the spirochetal forms Gram -ve were confirmed by microscopy (100x). From primeisolates and subsequent passages DNA was extracted for the molecular identification of specimens by the 16S rDNA-PCR. The occurrence of intestinal spirochetes isolation was 2.2% (5/225), of the positive samples, 4 were from Children Hospital of Tamaulipas and out of those, 2 from children of 9 and 14 months old, and the other 2 from children of 9 and 14 years of age. The results of this work showed evidence of intestinal spirochetes in fecal samples sent to the parasitology lab of the public hospitals included in this research. An important fact was the isolation of intestinal spirochetes from fecal samples of children, for which the mechanism of transmission remains uncertain. This work is the first report on the presence of the enteropathogen Brachyspira in the public health sector in Mexico.


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