Marrero RMT

Language: Spanish
References: 16
Page: 85-88
PDF size: 240.68 Kb.

ABSTRACT

The study of regulatory mechanisms mediated by micro ribonucleic acids has yielded encouraging results applicable in the diagnosis of cancer. Objective: To study the expression pattern of miR146b-5p and miR221-3p in thyroid carcinoma cell lines and its possible utility for the differential diagnosis of these types of tumors. Material and methods: An expression study of micro ribonucleic acids miR146b-5p and miR221-3p was performed in thyroid cancer cell lines BCPAD and 8505c. Reverse transcriptase polymerase chain reaction (PCR-RT) and quantitative reverse transcriptase PCR (PCR-RTq) assays were performed. The Mann-Whitney U test was used to compare the level of expression between cell lines (p ‹ 0.05). Significant differences were found in the expression of micro ribonucleic acids between the different cell lines, miR146b was significantly expressed in the BCPAD cell line, while the expression of miR221-3p was significantly higher in the 8505 cell line. Conclusions: Expression profiles of micro ribonucleic acids studied manifested themselves significantly in thyroid tumor cell lines, so they may constitute new biomarkers for the diagnosis of thyroid cancer.

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