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2020, Number 1

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Revista Habanera de Ciencias Médicas 2020; 19 (1)

Characterization of hyperimmune anti-human serum produced in sheep

Solana RJO, Reyes DLM, González RVE, Bosco LGMSJ, Borrego GY, Paradoa PML, Vega GI, Ramos PR, Pérez O

Language: Spanish
References: 24
Page: 30-39
PDF size: 750.30 Kb.


ABSTRACT

Introduction: Immunoelectrophoresis is a precipitation technique that allows the characterization of complex biological samples. The Immunology Department of the Institute of Basic and Pre-Clinical Sciences “Victoria de Girón” has a hyperimmune antiserum obtained by immunization of sheep against human serum total proteins and it also has an anti-human IgA antiserum obtained from human colostrum.
Objective: The aim of this study was to identify IgG, IgM and IgA in human serum and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.
Material and Methods: An observational descriptive cross-sectional study was conducted from November 2017 to June 2018. Immunoelectrophoresis of normal human serum was performed using hyperimmune antiserum.
Results: These procedures allowed to identify IgG, IgM and IgA in addition to albumin and other protein fractions and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.
Conclusions: This work allowed us to identify and determine significant anti-class responses of immunoglobulins in the sample studied.


REFERENCES

  1. Calderón V. Curso de técnicas de laboratorio en Inmunología. México: Universidad Autónoma de México; 2007.

  2. Ochoa R. Técnicas inmunoenzimáticas en el desarrollo clínico de vacunas. La Habana: Finlay Ediciones; 2013.

  3. Pérez O, Vega I. Inmunología en el humano sano. La Habana: Editorial Ciencias Médicas; 2017.

  4. Zacarias de Azevedo P, Sylvestre T, de Souza Cavalcante R, de Carvalho LR, Moris DV, Cotrim Sartor de Oliveira ML, et al. Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the diagnosis and dollow-up of patients with chronic pulmonary aspergillosis. Plos one [Internet]. 2015. [Citado 28/11/2017]. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC4536220/

  5. Lomonte B. Manual de Métodos Inmunológicos. 4ª ed. Costa Rica: Universidad de Costa Rica; 2007.

  6. Gutiérrez MIG, Castillo SZ, De la Torre Altamirano SP, Barillas MDU. Producción de sueros hiperinmunes contra los reguladores de crecimiento vegetal, ácido 2, 4- diclorofenoxiacético y kinetina. Revista Investigación Universitaria Multidisciplinaria [Internet]. 2013 Ene-Dic [Citado 01/10/ 2017] ; (12) :[164-70 p.] . Disponible en: https://dialnet.unirioja.es/servlet/articulo?c odigo=4745586

  7. González-Figueredo J, Rajme-Manzur D, Negrin-Thomson G, Dopico-Paz JR. Obtención de anticuerpos policlonales IgY antiparvovirus canino y evaluación en un sistema de aglutinación con látex. VacciMonitor [Internet]. 2015 [Cited 08/10/2017] ; 24(3):[7 p.]. Disponible en: http://scielo.sld.cu/scielo.php?pid=S102502 8X2015000300001&script=sci_arttext&tlng= pt

  8. Morris A. A short course on antibody production. En su: Radioisotopic and labelled reagent microanalytical methods used in medical research and clinical diagnosis. The British Council: University of Surrey; 1980. P.28.

  9. Özkan Ö, Aylan O, Ates C, Celebi B. Production of antirabies immune sera. Etlik Veteriner Mikrobiyoloji Dergisi. 2004;15:49-54.

  10. Redwan RM, Fahmy A, El Hanafy A, Abd El- Baky N, Sallam SM. Ovine anti-rabies antibody production and evaluation. Comp Immunol Microbiol Infect Dis. 2009;32:919.

  11. Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology. 9th ed. Barcelona: Elsevier; 2017.

  12. Delves P, Martin S, Burton D, Roitt I. Roitt's Essential Immunology. 13th ed. Chichester: Wiley Blackwell; 2017.

  13. Ouchterlony Ö. y Nilsson LÅ. Immunodiffusion and immunoelectrophoresis. En: Handbook of Experimental Immunology Immunochemistry. Oxford: Blackwell Scientific Publications; 1986.p.1- 32.

  14. Merlini G, Piro P, Pavesi F, Epis R, Aguzzi F. Detection and identification of monoclonal components: immunoelectrophoresis on agarose gel and immunofixation on cellulose acetate compared. 2001 p.1862-5.

  15. Altschuh D, Hennache G, Van Regenmortel MHV. Determination of IgG and IgM levels in serum by rocket immunoelectrophoresis using yolk antibodies from immunized chickens. J. Immunol. Method. 1984; 69:1-7.

  16. Grabar P, Burtin P. Analyse inmunoelectrophoretique.. París: Masson Cie; 1960.

  17. Hirshfeld J. Characterization of precipiting components in normal human sera obtained by an immunoelectrophoretic technique. Acta pathol. Microbiol. Schandinav. 1960; 49: 255.

  18. Praveen P, Jordan F, Priami C, Morine M. The role of breast-feeding in infant immune system: a systems perspective on the intestinal microbiome. Microbiome. 2015;3:1-12.

  19. Padrón S, Nieto Z. Determinación de IgA en leche materna en los quince días posparto. Revista de la Facultad de Ciencias Médicas Universidad de Cuenca; 2013;31:40-9.

  20. Jackson K, Nazar A. Breastfeeding, the Immune Response, and Long-term Health. J Am Osteopath Assoc. 2006;106:203-7.

  21. García R. Composición e inmunología de la leche humana. Acta Pediatr Mex. 2011;32:223-30.

  22. Calixto R, González M. Importancia clínica de la leche materna y transferencia de células inmunológicas al neonato. Perinatol Reprod Hum. 2011;25(2):109-14.

  23. Viazis S. Effects of High-Pressure Processing on Immunoglobulin A and Lysozyme Activity in Human Milk. JHL. 2011; 23:253-61.

  24. Palmeira P, Carneiro M. Immunology of breast milk. Rev Assoc Med Bras. 2016;62(6):584-93.




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