de la Puente LV, Cutiño JAM, López GT

Language: Spanish
References: 22
Page: 1-11
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ABSTRACT

Introduction: Brucella is a genus from the Brucellaceae family, Rhizobiales order. This genus is recognized for its high pathogenicity. Brucella bacteria cause brucellosis, a disease reported as one of the most important zoonoses worldwide due to its incidence in cattle and people. Previous studies on taxonomic classification of the genus have been mainly based on the analysis of gene 16S rDNA. However, few studies have been aimed at identification of molecular markers distinguishing its members from other groups of bacteria.
Objective: Identify insertions in preserved protein sequences which could be used as molecular markers for the taxonomy and diagnosis of species from the Brucella genus.
Methods: The homologous sequences for the proteins analyzed were obtained from international databases and aligned with the software ClustalX2, considering the parameters suggested in the literature.
Results: Insertions were identified in the proteins oxoglutarate dehydrogenase (component E1) and DNA ligase A, specific of the genus Brucella.
Conclusions: The insertions found may be used as complements to the traditional methods for taxonomic classification and for the molecular diagnosis of bacteria from the genus Brucella.

REFERENCES

1. Cárdenas FR, Herrera JKL. Revisión actualizada de la epidemiología de Brucelosis (Brucella abortus, Brucella mellitensis, Brucella suis, Brucella canis) en el Ecuador y el mundo. Centro de Biotecnología. 2018;6(1).

2. Mendoza O, Ramírez W, Yera G, Rosales Y, Mora E. La Brucelosis en bovinos de una provincia oriental de Cuba, en el período 2012-2014. REDVET Revista Electrónica de Veterinaria. 2015;16(5):1-11.

3. Obregón Fuentes AM, Muñoz Núñez K, Echevarría Pérez E, Rodríguez Olivera Y, Rodríguez Silveira J, Valdés Labrador Y, et al. Evaluación del sistema serológico Febrille Antigen Brucella para la pesquisa de anticuerpos contra brucelas, en Cuba. Revista Cubana de Medicina Tropical. 2015;67(3):0-.

4. Consortium U. UniProt: a worldwide hub of protein knowledge. Nucleic acids research. 2018;47(D1):D506-D15.

5. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids research. 1997;25(17):3389-402.

6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research. 1997;25(24):4876-82.

7. Hall BG. Comparison of the accuracies of several phylogenetic methods using protein and DNA sequences. Molecular biology and evolution. 2005;22(3):792-802.

8. Gupta RS. Protein Signatures Distinctive of Alpha Proteobacteria and Its Subgroups and a Model for α–Proteobacterial Evolution. Critical reviews in microbiology. 2005;31(2):101-35.

9. Naushad S. Discovery and applications of novel protein based molecular markers for bacterial classification and identification 2015.

10. Gupta RS. Impact of genomics on the understanding of microbial evolution and classification: the importance of Darwin's views on classification. FEMS microbiology reviews. 2016;40(4):520-53.

11. Wareth G, Melzer F, Neubauer H. In Brucella: Selective pressure may turn some genes on instead of default off position. Medical hypotheses. 2017;103:29-31.

12. McDonald W, Jamaludin R, Mackereth G, Hansen M, Humphrey S, Short P, et al. Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand. Journal of clinical microbiology. 2006;44(12):4363-70.

13. Colmenero J. Infecciones bacterianas crónicas (II). Brucelosis. Medicine-Programa de Formación Médica Continuada Acreditado. 2018;12(53):3124-31.

14. Oliveira Cavalcanti Soares CdP, Almeida Teles JA, Feitosa dos Santos A, Oliveira Firmino Silva S, Rocha Andrade Cruz MV, da Silva-Júnior FF. Prevalência da Brucella spp em humanos. Revista Latino-Americana de Enfermagem. 2015;23(5).

15. Díaz Herrera DF, Cruz Santana Y, Cruz Sui O, Martín Alfonso D, Alfonso González MJ, Ortiz Losada E, et al. Desarrollo y evaluación del desempeño de una prueba rápida inmunocromatográfica para el diagnóstico de la brucelosis. Revista de Salud Animal. 2015;37:105-11.

16. Obregón Fuentes AM, Cabrera Alvarado A, Echevarría Pérez E, Rodríguez Olivera Y, Rodríguez Silveira J. Detección de Brucella spp. por un sistema inmunocromatográfico comercial, en muestras ambientales cubanas. Revista Cubana de Medicina Tropical. 2015;67:0-.

17. Kuykendall L, Young J, Martínez-Romero E, Kerr A, Sawada H. Rhizobium. Bergey's Manual of Systematic Bacteriology. 2005:325-40.

18. Eskazan AE, Dal MS, Kaya S, Dal T, Ayyildiz O, Soysal T. Two cases of autoimmune hemolytic anemia secondary to brucellosis: a review of hemolytic disorders in patients with brucellosis. Internal Medicine. 2014;53(11):1153-8.

19. Khadka B, Adeolu M, Blankenship RE, Gupta RS. Novel insights into the origin and diversification of photosynthesis based on analyses of conserved indels in the core reaction center proteins. Photosynthesis research. 2017;131(2):159-71.

20. Ajawatanawong P, Baldauf SL. Evolution of protein indels in plants, animals and fungi. BMC evolutionary biology. 2013;13(1):140

21. Cutiño Jiménez AM, Barrera Roca L, de la Puente López V, Peña Cutiño HA. Molecular markers of insertion type in bacterias of the Moraxellaceae and Helicobacteraceae (phylum Proteobacteria) families. MediSan. 2018;22(01):40-7

22. Adeolu M, Alnajar S, Naushad S, Gupta RS. Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. International journal of systematic and evolutionary microbiology. 2016; 66(12):5575-99