2020, Number 4
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salud publica mex 2020; 62 (4)
Detection of Rickettsia typhi in Rhipicephalus sanguineus s.l. and Amblyomma mixtum in South of Mexico
Ulloa-García A, Dzul-Rosado K, Bermúdez-Castillero SE, López-López N, Torres-Monzón JA
Language: Spanish
References: 31
Page: 358-363
PDF size: 272.43 Kb.
ABSTRACT
Objective. To determine the presence of
Rickettsia typhi
in
Rhipicephalus sanguineus s.l. and
Amblyomma mixtum in
southern Mexico.
Materials and methods. Ticks were
collected in humans and domestic animals. The presence
of Rickettsia was determined by PCR and sequencing.
Results.
10/39 work vials amplified fragments of the
gltA, htrA
and
ompB genes. On 7/10 from
Rh. sanguineus s.l. collected
from dogs and in 3/10 of
A. mixtum collected from horse and
human. Sequencing indicated
R. typhi in
Rh. sanguineus and
A.
mixtum with 100% homology (LS992663.1) for a region of
the
htrA gene and 99% (LS992663.1) with the regions of the
gltA and
OmpB genes. The minimum infection rate (TMI) for
R. typhi was 3.88.
Conclusions. Rhipicephalus sanguineus s.l.
and
Amblyomma mixtum are naturally infected with
R. typhi
in Southern Mexico.
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