Morales GM, Bergues CLE, Camué CHM, Berenguer RC, Suárez PF, Rabionet JB, Betancourt HJE, Escalona AJC, Soria PNE, Mojena MY, Rodríguez CR, Mora TY, Arias PY, Sierra GVG

Language: Spanish
References: 21
Page: 1-19
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ABSTRACT

Introduction: Knowledge about the effects of electrochemotherapy on organisms may be important for the treatment of various diseases.
Objective: Evaluate the effects of low-intensity direct electric current on healthy mice.
Methods: Eighty healthy C57BL/6/Cenp mice (40 male and 40 female) were randomly distributed in eight experimental groups (four control and four treated). Direct electric current (10 mA during 5 min) or constant voltage (10 V during 5 min) was applied to the mice. Clinical, macroscopic and microscopic observation was performed, and measurements were taken of hematological and biochemical parameters of the blood.
Results: Reversible changes were induced in hematological, biochemical and histological parameters of the blood of male and female C57BL/6/Cenp mice when 10 mA or 10 V and various electrode arrays were used. These changes were less noticeable in the constant voltage mode.
Conclusions: The alterations induced by low-intensity direct electric current in healthy C57BL/6/Cenp mice are reversible. The systemic inflammatory process is dominated by the lymphocytes.

REFERENCES

1. Cury FL, Bhindi B, Rocha J, Scarlata E, El Jurdi K, Ladouceur M, et al. Electrochemical red-ox therapy of prostate cancer in nude mice. Bioelectrochemistry. 2015;104:1-9.

2. González MM, Morales DF, Cabrales LEB, Pérez DJ, Montijano JI, Castañeda ARS, et al. Dose-response study for the highly aggressive and metastatic primary F3II mammary carcinoma under direct current. Bioelectromagnetics. 2018;39(6):460-75.

3. Perkons NR, Stein EJ, Nwaezeapu C, Wildenberg JC, Saleh K, Itkin-Ofer R, et al. Electrolytic ablation enables cancer cell targeting through pH modulation. Communications biology. 2018;1(1):48.

4. Beheregaray WK, Gianotti GC, Leal JS, Garcez T, Contesini EA. Electrical stimulation in experimental wound healing in rabbits. Cienc Rural. 2014;44:878-83.

5. Phillips M, Rubinsky L, Meir A, Raju N, Rubinsky B. Combining electrolysis and electroporation for tissue ablation. Technol Cancer Res Treat. 2015;14(4):395-410.

6. Holandino C, Teixeira CAA, de Oliveira FAG, Barbosa GM, Siqueira CM, Messeder DJ, et al. Direct electric current treatment modifies mitochondrial function and lipid body content in the A549 cancer cell line. Bioelectrochemistry. 2016;111:83-92.

7. Zhou BG, Wei CS, Zhang S, Zhang Z, Shen YJ, Wang J, et al. Electrochemotherapy inhibited breast cancer MDA-MB231 cell migration and invasion through PI3K/AKT signal pathway. Int J Clin Exp Med. 2016;9(9):18513-8.

8. Calzado EM, Schinca H, Cabrales LEB, García FM, Turjanski P, Olaiz N, et al. Impact of permeabilization and pH effects in the electrochemical treatment of tumors: experiments and simulation. Appl Math Model. 2019;74: 62-72.

9. González MM. Daño tisular y cinética tumoral bajo la acción de la electroterapia con diferentes arreglos de electrodos. Tesis de Doctorado. Centro de Neurociencias: La Habana, Cuba. 2018 [acceso 19/07/2019]. Disponible en: https://www.researchgate.net/profile/Gustavo_Sierra2/publication/324673023.

10. Inui T, Amitani H, Kubo K, Kuchiike D, Uto Y, Nishikata T, et al. Case Report: A non-small cell lung cancer patient treated with gcmaf, sonodynamic therapy and tumor treating fields. Anticancer Res. 2016;36(7):3767-70.

11. Norma Española ISO 10993-2. Parte 2: Requisitos relativos a la protección de los animales. Septiembre; 1998.

12. Clark LH, Schein MW. Activities associated with conflict behavior in mice. Anim Behav. 1966;14(1):44-9.

13. Barbosa GM, dos Santos EG, Capella FNC, Homsani F, MarScal CP, Valle RS, et al. Direct electric current modifies important cellular aspects and ultrastructure features of Candida albicans Yeasts: influence of doses and polarities. Bioelectromagnetics. 2017;38(2):95-108.

14. Gaddam RR, Fraser R, Badiei A, Chambers S, Cogger VC, Le Couteur DG, et al. Differential effects of Kupffer cell inactivation on inflammation and the liver sieve following caecal-Ligation and puncture-induced sepsis in mice. Shock 2017;47(4):480-90.

15. Capellan O, Hollander JE, Pollack C Jr, Hoekstra JW, Wilke E, Tiffany B, et al. Prospective evaluation of emergency department patients with potential coronary syndromes using initial absolute CK-MB vs. CK-MB relative index. J Emerg Med. 2003;24(4):361-7.

16. Pupo AEB, González MM, Cabrales LEB, Navas JJG, Oria EJR, Jiménez RP, et al. 3D current density on tumor and surrounding healthy tissues generated by a system of straight electrode arrays. Math Comput Simul. 2017;138:49-64.

17. Soba A, Suarez C, González MM, Cabrales LEB, Pupo AEB, Reyes JB, et al. Integrated analysis of the potential, electric field, temperature, pH and tissue damage generated by different electrode arrays in a tumor under electrochemical treatment. Math Compt Simul. 2018;146:160-76.

18. Calzado EM, Rodríguez JLG, Cabrales LEB, García FM, Castañeda ARS, Delgado IMG, et al. Simulations of the electrostatic field, temperature and tissue damage generated by multiple-electrodes for electrochemical treatment. Appl Math Model. 2019;76:699-716.

19. Nanda BK. Electrotherapy simplified. New Delhi: Jaypee Brothers Medical Publishers; 2008. p. 98-106.

20. Keisari Y, Hochman I, Confino H, Korenstein R, Kelson I. Activation of local and systemic anti-tumor immune responses by ablation of solid tumors with intratumoral electrochemical or alpha radiation treatments. Cancer Immunol Immunother. 2014;63(1):1-9.

21. Gomes MN, Cardoso JS, Leitão AC, Holandino C. The mutagenic and genotoxic potential of the direct electric current in Escherichia coli and Salmonella thyphimurium Strains. Bioelectromagnetics. 2016;37:234-43.