2019, Number 1
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Rev Cubana Farm 2019; 52 (1)
Extraction, description and antioxidant activity of essential oil from Eucalyptus globulus Labill
Torrenegra AME, Granados CC, León MG
Language: Spanish
References: 20
Page: 1-12
PDF size: 497.07 Kb.
ABSTRACT
Introduction: Humans have used plants as sources of natural medicines and it is known that nowadays
approximately two-thirds of the population in developing countries uses them for the treatment of
diseases. Medicinal plants are characterized by having active substances that can modify the functioning
of biological systems by altering them or restoring organic functional balance.
Objectives: To evaluate the in vitro antioxidant activity of the Eucalyptus globulus Labill´s essential oil grown in the municipality of Pamplona-Norte de Santander, Colombia.
Methods: Essential oil was extracted from the leaves by hydrodistillation and assisted hydrodistillation
by radiation with microwave. It was determined a relative density to 20°C, refractive index, solubility of
the essential oils in ethanol (70% v/v) and optical rotation. Chemical composition was assessed using
gas chromatography/mass spectrometer. The antioxidant activity was determined using the techniques of
anti-free radicals activity by DPPH .and ABTS.+ methods.
Results: Yields ranged from 0.72 to 1.21% depending on the extraction method used. The results of the
test of antioxidant activity showed that the essential oils of Eucalyptus globulus Labill obtained by both
methods of extraction had promising results; in addition, these oils presented high levels of monoterpenes
with recognized antioxidant activity, such as eucalyptol.
Conclusions: The essential oil of Eucalyptus globulus Labill is considered as promising to design master
products with antioxidant activity.
REFERENCES
Matiz G, Osorio MR, Camacho F, Atencia M, Herazo J. Diseño y evaluación in vivo de fórmulas para acné basadas en aceites esenciales de naranja (Citrus sinensis), albahaca (Ocimum basilicum L) y ácido acético. Biomédica: Revista del Instituto Nacional de Salud. 2012;32(1):125-133.
Granados C., Yáñez Y, Santafé G. Evaluación de la actividad antioxidante del aceite esencial foliar de Calycolpus moritzianus y Minthostachys mollis de Norte de Santander. Bistua: Revista de la Facultad de Ciencias Básicas. 2012;10(1):12-23.
Shankar R & Mohan S. A status review on the medicinal properties of essential oils. 2014;62:250-264.
Stashenko E, Ruíz CA, Arias G, Durán DC, Salgar W, Cala M, Martínez MR. Lippia origanoides chemotype differentiation based on essential oil GC-MS analysis and PCA. J. Sep. Sci. 2010;33 93-103.
Matiz G, Fuentes K, León G. Microencapsulación de aceite esencial de tomillo (Thymus vulgaris) en matrices poliméricas de almidón de ñame (Dioscorea rotundata) modificado. Rev. Colomb. Cienc. Quím. Farm. 2015;44(2):189-207.
Hoda F, Friedheelm M., Abdalla E, Ahmed E. Effect of extraction techniques on the chemical composition and antioxidant activity of Eucalyptus camaldulensis var. brevirostris leaf oils. Zeitschrift für Lebensmitteluntersuchung und -Forschung A. 1999;208:212-216.
Granados C, Santafé GG, Acevedo D. Chemical composition and evaluation of antioxidant activity of leaf essential oil Eucalyptus camaldulensis from Norte de Santander (Colombia). Rev. U.D.C.A Act. & Div. Cient. 2015;18(1):235-240.
Ferhat AM, Meklati YB, Smadja J, Chemat F. An improved microwave Clevenger apparatus fos destillation of essential oils from orange peel. Journal of Chromatography A. 2006;1112:121-126.
Golmakani MT, Rezaei K. Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils fron Thymus vulgaris L. Food Chemistry. 2008;109:925-930.
Torrenegra M, Granados C, Osorio M, León G. Method comparison of hydrodistillation microwave radiation-assisted (MWHD) front hydrodistillation (HD) in the extraction of essential oil of Minthostachys mollis. Inf. Tecnol. 2015;26(1):117-122.
Torrenegra M, Matiz G, León G, Gil J. Actividad antibacteriana in vitro de aceites esenciales frente a microorganismos implicados en el acné. Revista cubana de Farmacia. 2015 [acceso 01/07/2019];49(3). Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034- 75152015000300011&lng=es
León G, Osorio MR, Martínez SR. Comparación de dos métodos de extracción del aceite esencial de Citrus Sinensis L. Revista Cubana de Farmacia. 2015 [acceso 01/07/2019];49(4). Disponible en: http://scielo.sld.cu/pdf/far/v49n4/far14415.pdf
Baharum SN, Bunawan H, Ghani MaA, Mustapha WAW, Noor NM. Analysis of the chemical composition of the essential oil of Polygonum minus Huds. using two-dimensional gas chromatographytime- of-flight mass spectrometry (GC-TOF MS). Molecules. 2010;15(10):7006-15.
Formisano C, Oliviero F, Rigano D, Saab AM, Senatore F. Chemical composition of essential oils and in vitro antioxidant properties of extracts and essential oils of Calamintha origanifolia and Micromeria myrtifolia, two Lamiaceae from the Lebanon flora. Industrial Crops and Products. 2014;62:405-411.
Matiz G, Osorio M, León G. Actividad antibacteriana in vitro de diecinueve aceites esenciales frente a bacterias asociadas al acné. Revista Cubana de Farmacia. 2015 [acceso 01/07/2019];49(1). Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034- 75152015000100011&lng=es&tlng=es
Silva B, Andrade P, Valentao P, Ferreres F, Seabra R., Ferreira M. Quince (Cydonia oblonga Miller) Fruit (Pulp, Peel, and Seed) and Jam: Antioxidant Activity. J. Agric. Food Chem. 2004;52:4705-4712.
Naranjo M, Velez LT, Rojano BA. Antioxidant activity of different grades of Colombian coffee. Rev Cubana Plant Med. 2011;16:164-173.
Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem. 2005;53:4290-4302.
Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis. 2011;24(7):1043-1048.
Rincón CA, Castaño JC, Ríos E. Actividad biológica de los aceites esenciales de Acmella ciliata (Kunth) Cass. Rev Cubana Plant Med. 2012;17(2):160-171.