Martínez MSM, Jiménez MMC, García VPE, Figueroa LM, Maceiras CMA

Language: Spanish
References: 19
Page: 166-176
PDF size: 271.57 Kb.

ABSTRACT

Introduction: Plant species are the first remedy to the people's health problems. The pomegranate is rich in ascorbic acid, improves the epidermal barrier and reduces the contraction of wounds. The water hyacinth has antibacterial activity.
Objective: To evaluate the healing effect of the charred powder of Punica granatum Linn (pomegranate) and Eichhornia crassipes (water hyacinth), in an experimental model in rats.
Methods: Experimental analytical study with 30 male rats distributed in 3 groups (n = 10). Group I and II; treated with the charred powder of the pomegranate and water hyacinth respectively. Group III: treated with 0.9 % sodium chloride. An incision wound model was made on the back. The evolution of healing was followed by the speed of contraction of the wound in millimeters. Histology was performed on biopsies of scar tissue. For the statistical analysis, the nonparametric Mann-Whitney test was used. The level of significance was set at p < 0.05.
Results: The healing effect of the charred powders of the pomegranate and water hyacinth was verified; decreased the wound area significantly compared to the control group. The histological study showed mature grade III dermis in groups I and II.
Conclusions: The topical application of the charred powder of pomegranate and water hyacinth influenced the closure of the wounds and the maturation of the dermis, which favored healing.

REFERENCES

1. Lantzouraki Dimitra Z, Sinanoglou VJ, Zoumpoulakis P, Proestos C. Comparison of the Antioxidant and Antiradical Activity of Pomegranate (Punica granatum L.) by Ultrasound-Assisted and Classical Extraction. Analytical Letters. 2016;49(7):969-978.

2. Weixi L. Pomegranate (Punica granatum) fruit polyphenols, punicalagin, ellagic acid and gallic acid, inhibit formation of advanced glycation end products by scavenging reactive carbonyl species. The FASEB Journal. 2014; 28(1):975-10.

3. Sepúlveda RB, Haro IR, Castillo MS. Efecto del extracto hidroalcohólico de Punica granatum sobre la viabilidad de Staphylococcus aureus y Pseudomonas aeruginosa "in vitro". Revista Científica de Estudiantes. 2014; 2(1):23-31.

4. Minghui F, Lihua J, Yuanmei L, Guohua Y, Lijun Z, Jinping P. Identification of gene fragments related to nitrogen deficiency in Eichhorniacrassipes (Pontederiaceae). Revista de Biología Tropical. 2014; 62(4):1637-48.

5. García Valido PE, Pérez Alejo JL. Mangifera Indica, L. Especies medicinales en el Delta Orinoco: aspectos promisorios para la medicina tradicional cubana. La Habana: ECIMED; 2011.

6. Ministerio de salud Pública (Cuba). Normas Ramales de Medicamentos de origen vegetal. Tinturas y extractos fluidos. La Habana: MINSAP; 1992.

7. De la Rosa-Belmonte SJ, López-Carmen F, Ramírez-Hernández JE, Sánchez-Núñez OJ, Guerrero-Arenas R. Consideraciones éticas en el manejo de animales en campo y en laboratorio. Ciencia y Mar. 2013; 17(49):45-54.

8. Velasquez RAC. Declaración de Helsinki de la Asociación Médica Mundial-Principios éticos para las investigaciones médicas en seres humanos. Seúl, octubre de 2008. Journal of Oral Research. 2013; 2(1):42-4.

9. González-Quevedo Rodríguez M, Abin G, Wolt N, Batista M, Ramos I. Quemaduras dérmicas tratadas con una crema de Aloe barbadensis Miller cultivada en Cuba. Estudio Experimental. La Habana: ISMM, MINFAR; 1990.

10. Tillán Capo Juana I, Castro Méndez Irma, Bueno Pavón Viviana, Carrillo Domínguez Carmen, Ortiz Infante Melba. Efecto cicatrizante de la crema de extracto etanólico de cera de caña. Rev Cubana Plant Med. 2004 Ago;9(2):[aprox. 6 p.] Acceso: 16/01/2019. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962004000200002&lng=es

11. Robalino López CE. Evaluación del efecto antidiarreíco y cicatrizante de la infusión y del extracto etanólico de Cyclospermun leptophyllum (Pers.) Sprague en ratones (Mus musculus) y conejos (Oryctologus cuniculus) [Tesis de bachiller]: Escuela Superior Politécnica de Chimborazo. Facultad de Ciencias. Escuela de Bioquímica y Farmacia; 2014. Acceso: 16/01/2019. Disponible en: http://dspace.espoch.edu.ec/bitstream/123456789/3314/1/56T00439.pdf

12. Janani J, Lydia ED. Total polyphenol content and minimum inhibitory concentration of pomegranate (Punica granatum Linn) extracts against oral microorganisms. StomatološkiglasnikSrbije. 2013; 60(4):183-90.

13. Zarei M, Azizi M, Bashir-Sadr Z. Evaluation of physicochemical characteristics of pomegranate (Punica granatum L.) fruit during ripening. Fruits. 2011; 66(2):121-9.

14. Vivot EP, Sánchez C, Cacik F, Sequin C. Actividad antibacteriana en plantas medicinales de la flora de Entre Ríos (Argentina). Ciencia, docencia y tecnología. 2012; (45):131-46.

15. Lantzouraki DZ, Sinanoglou VJ, Zoumpoulakis P. Proestos C. Comparison of the Antioxidant and Antiradical Activity of Pomegranate (Punica granatum L.) by Ultrasound-Assisted and Classical Extraction. Analytical Letters. 2016;49(7): 969-78.

16. Domínguez Suárez A, Acosta Ulloa L, Cuello D. Efecto cicatrizante de extracto fluido de hojas de Siempreviva. Rev Cubana Plant Med. 2001; 6(1):16-8.

17. González Tuero JH. Efecto del limo de la salina de Guantánamo en la cicatrización por segunda intención en ratas. MEDISAN. 2014; 18 (9):1195-203.

18. Charas Dos Santos IF, Nhambirre A, Bata Bambo O, Mota Cardoso JM, Celeita Rodríguez N, López Castañeda BS. Cissus quadrangularis (Linn) (Uva Velve) y Phyllanthus reticulatus Poir (Linn) (Pancolin) en la cicatrización de heridas. Rev Cubana Plant Med. 2015; 20(3):348-57.

19. Vizcaíno César M, Alarcón Arango I, Sebazco Perna C, Maceira Cubiles MA. Importancia de la sacarosa para la cicatrización de heridas infectadas. Rev Cub Med Mil. 2013;42(1):49-55.