medigraphic.com
ISSN 0301-696X (Print)
Órgano de difusión del Servicio de Sanidad Militar y del Colegio Nacional de Médicos Militares

2019, Number 3-4

Next >>

Rev Sanid Milit Mex 2019; 73 (3-4)

Study of the transcriptional regulation of breast cancer in Mexican women with overweight and obesity by microarrays

Floriano-Sánchez E, Ignacio-Mejía I, Brindis-Hernández FG, Córdova-Espinoza MG, Romero-Morelos P, Quintero-Fabián S, Comoto-Santacruz DA, Estrada NLE, Heredia-Caballero ÁG, Millán-Juárez Á, Ortiz-León JL, Arroyo MIM, Cárdenas-Rodríguez N

Language: Spanish
References: 21
Page: 193-200
PDF size: 344.56 Kb.


ABSTRACT

Breast cancer is the leading cause of death in women. Currently, the signalling pathways and genes involved in carcinogenesis, as well as their relationship to adipose tissue and their consequent association with poor prognosis are not fully described. The objective was to identify key genes of the tumour process in breast cancer in overweight and obese Mexican women. Using microarrays, the transcriptional regulation of breast cancer tumour tissue was compared with that of the adjacent non-tumour tissue of the mammary gland. Subsequently, the bioinformatics analysis identified 174 genes with fold change ≥ or ≤ 2 and a p ≤ 0.05. 109 genes were overexpressed and 65 underexpressed in breast cancer tumor tissue in association with excess adipose tissue. This shows that in breast cancer tumour tissue associated with obesity there is deregulation of genes that modify the process of apoptosis. We propose that the HAAO gene involved in the process of NAD synthesis is key in the regulation of the NAD+/-SIRT1-p53 axis, and also highlight the impact of the overexpression of the RPS6KB2 gene in the FSH hormone signaling pathway.


REFERENCES

  1. Ghoncheh M, Pournamdar Z, Salehiniya H. Incidence and mortality and epidemiology of breast cancer in the world. Asian Pac J Cancer Prev. 2016; 17: 43-46.

  2. Anderson BO, Cazap E, El Saghir NS, Yip CH, Hussein MK, Isabel VO et al. Optimization of breast cancer management in low-resource and middle-resource countries: executive summary of the Breast Health Global Initiative consensus, 2010. Lancet Oncol. 2011; 12 (4): 387-398.

  3. Soto-Perez-de-Celis E, Chavarri-Guerra Y. National and regional breast cancer incidence and mortality trends in Mexico 2001-2011: analysis of a population-based database. Cancer Epidemiol. 2016; 41: 24-33.

  4. Maffuz-Aziz A, Labastida-Almendaro S, Espejo-Fonseca A. Características clinicopatológicas del cáncer de mama en una población de mujeres en México. Cir Cir. 2017; 85 (3): 3-9.

  5. Gui Y, Pan Q, Chen X, Xu S, Luo X, Chen L. The association between obesity related adipokines and risk of breast cancer: a meta-analysis. Oncotarget. 2017; 8: 75389-75399.

  6. Renehan AG, Tyson M, Egger M, Heller RF, Zwahlen M. Body-mass index and incidence of cancer: a systematic review and meta-analysis of prospective observational studies. Lancet. 2008; 371: 569-578.

  7. Hernández-Mesa N. Mecanismos del tránsito de la obesidad al cáncer. Rev CENIC Ciencias Biol. 2016; 47 (1): 33-50.

  8. Lockhart DJ, Winzeler EA. Genomics, gene expression and DNA arrays. Nature. 2000; 405 (6788): 827-836.

  9. Lebeau A, Kriegsmann M, Burandt E, Sinn HP. Invasive breast cancer: the current WHO classification. Pathologe. 2014; 35 (1): 7-17.

  10. Cantó C, Menzies K, Auwerx J. NAD+ metabolism and the control of energy homeostasis - a balancing act between mitochondria and the nucleus. HHS Public Access. 2015; 22 (1): 31-53.

  11. Yi J, Luo J. SIRT1 and p53, effect on cancer, senescence and beyond. Biochim Biophys Acta. 2010; 1804 (8): 1684-1689.

  12. Sharif T, Ahn DG, Liu RZ, Pringle E, Martell E, Dai C et al. The NAD+ salvage pathway modulates cancer cell viability via p73. Cell Death Differ. 2016; 23 (4): 669-680.

  13. Behrouzfar K, Alaee M, Nourbakhsh M, Gholinejad Z, Golestani A. Extracellular NAMPT/visfatin causes p53 deacetylation via NAD production and SIRT1 activation in breast cancer cells. Cell Biochem Funct. 2017; 35 (6): 327-333.

  14. Yang H, Yan B, Liao D, Huang S, Qiu Y. Acetylation of HDAC1 and degradation of SIRT1 form a positive feedback loop to regulate p53 acetylation during heat-shock stress. Cell Death Dis. 2015; 6: 1747.

  15. Planeix F, Siraj MA, Bidard FC, Robin B, Pichon C, Sastre-Garau X et al. Endothelial follicle-stimulating hormone receptor expression in invasive breast cancer and vascular remodeling at tumor periphery. J Exp Clin Cancer Res. 2015; 34 (1): 12.

  16. Sarmento-Cabral A, Peinado JR, Halliday LC, Malagon MM, Castaño JP, Kineman RD et al. Adipokines (leptin, adiponectin, resistin) differentially regulate all hormonal cell types in primary anterior pituitary cell cultures from two primate species. Sci Rep. 2017; 7: 43537.

  17. Hosney M, Sabet S, El-Shinawi M, Gaafar KM, Mohamed MM. Leptin is overexpressed in the tumor microenvironment of obese patients with estrogen receptor positive breast cancer. Exp Ther Med. 2017; 13 (5):2235-2246.

  18. Karlsson E, Pérez-Tenorio G, Amin R, Bostner J, Skoog L, Fornander T et al. The mTOR effectors 4EBP1 and S6K2 are frequently coexpressed, and associated with a poor prognosis and endocrine resistance in breast cancer: A retrospective study including patients from the randomised Stockholm tamoxifen trials. Breast Cancer Res. 2013; 15 (5): R96.

  19. Basu A, Sridharan S. Regulation of anti-apoptotic Bcl-2 family protein Mcl-1 by S6 kinase 2. PLoS One. 2017; 12 (3): e0173854.

  20. Boulay PL, Louise M, Turpin J, Huot-Marchand JE, Lavoie C, Sanguin-Gendreau V et al. Rab11-FIP1C is a critical negative regulator in ErbB2-mediated mammary tumor progression. Cancer Res. 2016; 76 (9): 2662-2674.

  21. Balda MS, Garrett MD, Matter K. The ZO-1-associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. J Cell Biol. 2003; 160 (3): 423-432.




2020     |     www.medigraphic.com