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2019, Number 1

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AbanicoVet 2019; 9 (1)

Effect of different Beauveria bassiana strains against Alphitobius diaperinus from poultry farms in the state of Colima

Moreno-Borjas M, Prado-Rebolledo O, García-Casillas A, Ángel-Sahagún A, Sánchez-Chiprés D, García-Márquez L

Language: Spanish
References: 24
Page: 1-8
PDF size: 607.87 Kb.


ABSTRACT

The aim of this study was to evaluate the effect of different Beauveria bassiana strains (B. bassiana) against Alphitobius diaperinus (A. diaperinus). A total of 360 beetles A. diaperinus (adults) were collected from poultry farms in Colima state. After verifying its viability (90% of germinated conidia), five of B. bassiana strains (Bb3, Bb4, Bb5, Bb6 and Bb17) were used. The five treatments of B. bassiana, presented a concentration of 1 x 108 conidia/mL. Fifteen beetles were used for the control and for each of B. bassiana strain, with four replicates/treatment. The dead A. diaperinus were incubated for 21 d, to promote mycelial growth and thus determine whether the death was caused by B. bassiana. The resulting data set was analyzed by descriptive statistics. The comparison between groups was assessed by Analysis of Variance and frequency histogram. The results showed that all fungal isolates were pathogenic against A. diaperinus beetles. The Bb4 strain showed a difference between groups, with respect to mortality (5.25 ± 0.95 dead A. diaperinus beetles). In dead beetles, mycosis due to mycelium growth of B. bassiana, presented the best results in Bb4 strain (2.50 ± 1.29 beetles A. diaperinus with mycosis) equivalent to 16.66%ith the lowest value in Bb3 strain and Bb6 strain (3.33 and 1.66% of mycosis) respectively. The results found could be used in future trials to evaluate the Bb4 strain under normal conditions within the bird facilities.


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