2019, Number 1
Determination of non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol and cholesterol during negative energy balance in Holstein cows
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ABSTRACT
The aim of this study was to quantify the concentration of non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol and cholesterol during the negative energy balance in Holstein cows. The study was carried out by sampling and analyzing 250 blood serums obtained from 50 Holstein cows. After calving, the first sample was performed at 7 d, the second at 15 d and the remaining three at 30, 45 and 60 d. Mean values for non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol, and cholesterol were calculated. The resulting data set was analyzed by Gaussian distribution and descriptive statistics. Confidence intervals of 95% were established. The comparison between groups was assessed by Analysis of Variance and frequency histogram. The non-esterified fatty acids, β-hydroxybutyrate, and triacylglycerol are consistent with values reported by international literature. Higher values were found for cholesterol. The AGNE did not present differences between groups. The β-hydroxybutyrate showed its highest concentration at 15 d postpartum and the lowest at 60 d postpartum. Triacylglycerol and cholesterol showed their highest concentration at 60 d postpartum, and the lowest at 15 d postpartum. The calculated confidence intervals could be used at herd level to detect alert situations when at least 5 % of the sampled cows would fall outside of the calculated reference interval for a given parameter.REFERENCES
ABDELLI A, Raboisson D, Kaidi R, Ibrahim B, Kalem A, Iguer-Ouada M. 2017. Elevated non-esterified fatty acid and β-hydroxybutyrate in transition dairy cows and their association with reproductive performance and disorders: a meta-analysis. Theriogenology. 93(1):99-104. ISSN: 1879-3231 (Electronic) 0093-691X (Linking). DOI: 10.1016/j.theriogenology.2017.01.030.
DA FONSECA LLF, Rodríguez MPH, dos Santos VM, Lima PA, Lucci SC. 2004. Supplementation of dairy cows with propylene glycol during the periparturient period: effects on body condition score, milk yield, first estrus post-partum, β-hydroxybutyrate, non-esterified fatty acids and glucose concentrations. Cienc Rural. 34(3):897-903. ISSN: 1678-4596 (Electronic). DOI: 10.1590/S0103-84782004000300037.
DUSKE K, Hammon HM, Langhof AK, Bellmann O, Losand B, Nurnberg K, Nurnberg G, Sauerwein H, Seyfert HM, Metges CC. 2009. Metabolism and lactation performance in dairy cows fed a diet containing rumen-protected fat during the last twelve weeks of gestation. J Dairy Sci. 92(4):1670-1684. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2008-1543.
FOLNOŽIĆ I, Turk R, Đuričić D, Vince S, Pleadin J, Flegar-Meštrić Z, Valpotić H, Dobranić T, Gračner D, Samardžija M. 2015. Influence of Body Condition on Serum Metabolic Indicators of Lipid Mobilization and Oxidative Stress in Dairy Cows During the Transition Period. Reprod Domest Anim. 50(6):910-917. ISSN: 1439-0531 (Electronic) 0936-6768 (Linking). DOI: 10.1111/rda.12608.
MANI O, Sorensen MT, Sejrsen K, Bruckmaier RM, Albrecht C. 2009. Differential expression and localization of lipid transporters in the bovine mammary gland during the pregnancy-lactation cycle. J Dairy Sci. 92(8):3744-3756. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2009-2063.
MANI O, Korner M, Ontsouka CE, Sorensen MT, Sejrsen K, Bruckmaier RM, Albrecht C. 2011. Identification of ABCA1 and ABCG1 in milk fat globules and mammary cells-- implications for milk cholesterol secretion. J Dairy Sci. 94(3):1265-1276. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2010-3521.
RUOFF J, Bertulat S, Burfeind O, Heuwieser W. 2016. Associations of β-hydroxybutyrate, cholesterol, triglycerides and high-density lipoproteins to non-esterified fatty acids preand postpartum. J Dairy Res. 83(4):447-452. ISSN: 1469-7629 (Electronic) 0022-0299 (Linking). DOI: 10.1017/S0022029916000534.
SHEEHY MR, Fahey AG, Aungier SPM, Carter F, Crowe MA, Mulligan FJ. 2017. A comparison of serum metabolic and production profiles of dairy cows that maintained or lost body condition 15 days before calving. J Dairy Sci. 100(1):536-547. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2016-11206.
SUN F, Cao Y, Cai C, Li S, Yu C, Yao J. 2016. Regulation of nutritional metabolism in transition dairy cows: energy homeostasis and health in response to post-ruminal choline and methionine. PLoS One. 11(8):e0160659-e0160669. ISSN: 1932-6203 (Electronic) 1932-6203 (Linking). DOI: 10.1371/journal.pone.0160659.
VAN HOEIJ RJ, Dijkstra J, Bruckmaier RM, Gross JJ, Lam T, Remmelink GJ, Kemp B, van Knegsel ATM. 2017. The effect of dry period length and postpartum level of concentrate on milk production, energy balance, and plasma metabolites of dairy cows across the dry period and in early lactation. J Dairy Sci. 100(7):5863-5879. ISSN: 1525- 3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2016-11703.