medigraphic.com
ISSN 1870-199X (Print)
Órgano oficial de la Facultad de Odontología, UNAM

2006, Number 2

<< Back Next >>

Rev Odont Mex 2006; 10 (2)

In vitro evaluation in the cytotoxicyty of three endodontic sealers on mice fibroblasts

Peralta PM, Uribe QE, García ARL, Gutiérrez OG

Language: Spanish
References: 20
Page: 63-68
PDF size: 217.67 Kb.


ABSTRACT

Endodotic sealers may cause periapical tissue cytotoxicyty following root canal treatments. In general, in vitro biocompatibility tests evaluate the cytotoxicity of sealers long after they have hardened. Hence, in this study we evaluated the acute effects of three sealers: AH-PlusTM, RoekoSealAutomix® (RSA) y Roth-Root using a pore membrane system where the sealers are placed on top just after mixing. Mouse fibroblast cultures were exposed for 6, 12, 24, 36, 48 y 72 hours to the sealers described above. After each period, the morphology and viability of fibroblasts were evaluated. There were no differences in morphology between cells exposed to RSA and control conditions. Fibroblasts exposed to the other two sealers detached from the culture dish, lost their flame-like morphology and appeared rounded. The number of cells in cultures exposed to RSA was similar to that of the control cultures. In contrast, fibroblast exposed to the other two sealers died after 6 hours of culture. In conclusion, because RSA essentially showed no cytotoxicyty, it appears that silicon-based sealers are the best options for treating root canals.


REFERENCES

  1. Schwarze T, Leeyhausen G, Geurtsen W. Long-term cytocompatibility of various endodontic sealers using a new root canal model. J Endodon 2002; 28 (11): 749-753.

  2. Pertot WJ, Stephan G, Tardieu C, Proust JP. Comparison of the intraosseous biocompatibility of Dyract and Super EBA. J Endodon 1997; 23: 315-9.

  3. Nyguen TN. Obturation of root canal system. In Pathways of the pulp. 5th ed. (eds. S. Cohen and Burns) C.V. Mosby Co. St. Louis, MO. USA. 1991: 193.

  4. Sjogren U, Sundqvist G, Nair PN. Tissue reaction to gutta-percha particles of various sizes when implanted subcutaneously in guinea pigs. Eur J Oral Sci 1995; 103: 313-321.

  5. Kamran E. Safavi DMD, Larz SW, Spangberg DDS, Ney S, Costa George Sapounas DDS. An in vitro Method for Longitudinal Evaluation of Toxicity of Endodontic Sealers. 1989; 15: 484-6.

  6. ANSI/ADA Specification 41 for biological evaluation of dental materials. Approved January 4, 1982.

  7. Osario RM, Fet A, Vertucci F, Shawley AL. Cytotoxicity of endodontic materials. J Endodon 1998; 24: 91.

  8. Vajrabhaya L, Sithisarn P. Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers. Int Endod J 1997; 30: 141-4.

  9. Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide- based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002; 35: 153-168.

  10. Koulaouzidou EA, Papazisis KT, Beltes P, Geromichalos GD, Kortsaris AH. Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation. Endod Dent Traumatol 1998; 14: 182-185.

  11. Current Protocols in Cell Biology. Chapter 2: Assessment of Cell Toxicity. Basic Protocol 1: Tripan Blue Uptake to Assess Viability.

  12. Rappaport HM, Lilly GE, Kapsimalis P. Toxicity of endodontic filling materials. Oral Surg, Oral Med, Oral Pathol 1964; 8: 785-802.

  13. Nakamura H, Matsumoto Y, Hirano S et al. Study on the cytotoxicity of root canal filling materials. J Endodon 1986; 12: 156-160.

  14. Cohen B, Pagnillo MK et al. An in vitro Study of the Cytotoxicity of Two Root Canal Sealers. J Endodon 2000; 26(4): 228-229.

  15. Molina-Guarneros JA, García-Cuéllar G, Martínez-Valenzuela A, García-Mondragón MJ, Llamosas-Hernández E, García-Aranda RL, Arzate H. Effects of six endodontic cements on the cells of the murine immune system in vitro. Proc West Pharmacol Soc 1998; 41: 47-50.

  16. Nasim GA, Mahnaz H, Zahra SB, Fazel S. In vitro cytotoxicity of a new epoxy resin root canal sealer. J Endodon 2000; 26(8): 462-465.

  17. Schwarze T, Leyhausen G, Geurtsen W. Long-Term cytocompatibility of various endodontic sealers using a new root canal model. J Endodon 2002; 28(11): 749-753.

  18. Dartar MO, Yilmaz, Kalayci A, Zaímoglu L. A comparison of the in vitro cytotoxicity of two root canal sealers. J Oral Rehab 2003; 30: 426-429.

  19. Min-Kai W, Tigos E, Wesselink PR. An 18-month longitudinal study on a new silicon-based sealer, RSA RoekoSeal: A leakage study in vitro. Oral Surg, Oral Med, Oral Pathol 2002; 94: 499-502.

  20. Gencoglu N, Turkmen C, Ahiskali R. A new silicon-based root canal sealer (Roekoseal-Automix). J Oral Rehabil 2003; 30(70): 753-7.




2020     |     www.medigraphic.com