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2019, Number 4

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Vet Mex 2019; 6 (4)

Molecular characterization of partial and nearly full parvovirus VP2 gene sequences from Turkish domestic cats

Akkutay-Yoldar Z

Language: English/Spanish
References: 31
Page: 1-12
PDF size: 983.82 Kb.


ABSTRACT

Parvoviruses are ubiquitous pathogens that cause a fatal disease in cats and are able to mutate for cross-species transmission. Both the feline panleukopenia virus (FPV) and the canine parvovirus (CPV), with their antigenic variants, induce a disease in cats that presents with similar signs. The aim of this study was to determine the presence of parvoviruses in blood and exudate samples from five clinically symptomatic cats (from Ankara, Turkey). The gene coding for the VP2 structural capsid protein of the obtained parvoviruses was amplified by polymerase chain reaction (PCR), purified and partially or nearly full-length sequenced. The maximum likelihood (ML) method was used for molecular characterization. Phylogenetic analysis based on nearly full-length sequencing of the VP2 gene and amino acid arrangement showed that four of the viral strains were closely related and localized in the same FPV cluster. The fifth strain found was located in the same cluster but on a separate branch. Viral field strains were included in the CPV-2 group as determined by partial genome analysis: four fitted in the CPV-2c, and one in a separate clade within the CPV-2b group. To our knowledge, this is the first report that details nearly full-length VP2 gene characterisation in Turkish cats. Overall, nearly full-length VP2 contrasts were more effective to determine the origin of parvovirus strains, than partial length comparisons.


REFERENCES

  1. Allison AB, Kohler DJ, Ortega A, Hoover EA, Grove DM, Holmes EC, et al. Host-specific parvovirus evolution in nature is recapitulated by in vitro adaptation to different carnivore species. PLoS Pathog. 2014;10:e1004475.

  2. Decaro N, Buonavoglia C. Canine parvovirus—A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet. Microbiol. 2012;155:1-12.

  3. Greene CE. Infectious diseases of the dog and cat. E-book. 4th ed. St. Louis, MO: Elsevier Health Sciences; 2013.

  4. Battilani M, Scagliarini A, Ciulli S, Morganti L, Prosperi S. High genetic diversity of the VP2 gene of a canine parvovirus strain detected in a domestic cat. Virology. 2006;352:22-6.

  5. Ohshima T, Mochizuki M. Evidence for recombination between feline panleukopenia virus and canine parvovirus type 2. J Vet Med Sci. 2009;71:403-8.

  6. Cságola A, Varga S, Lőrincz M, Tuboly T. Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary. Arch Virol. 2014;159:2441-4.

  7. Mochizuki, M, Ohshima, T, Une, Y, and Yachi, A, Recombination between vaccine and field strains of canine parvovirus is revealed by isolation of virus in canine and feline cell cultures, J. Vet. Med. Sci, 2008, vol. 70, pp. 1305-1314.

  8. Yang S, Wang S, Feng H, Zeng L, Xia Z, Zhang R, et al. Isolation and characterization of feline panleukopenia virus from a diarrheic monkey. Vet Microbiol. 2010;143:155-9.

  9. Truyen U, Parrish, CR. Canine and feline host ranges of canine parvovirus and feline panleukopenia virus: distinct host cell tropisms of each virus in vitro and in vivo. J Virol. 1992;66:5399-408.

  10. Foley JE, Orgad U, Hirsh DC, Poland A, Pedersen NC. Outbreak of fatal salmonellosis in cats following use of a high-titer modified-live panleukopenia virus vaccine. J Am Vet Med Assoc. 1999;214:67-70.

  11. Kruse BD, Unterer S, Horlacher K, Sauter-Louis C, Hartmann K. Prognostic factors in cats with feline panleukopenia. J Vet Int Med. 2010;24:1271-6.

  12. Steinel A, Munson L, Van Vuuren M, Truyen U. Genetic characterization of feline parvovirus sequences from various carnivores. J Gen Virol. 2000;81:345-50.

  13. Muz D, Oğuzoğlu TÇ, Timurkan MÖ, Akın H. Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey. Virus Genes. 2012;44:301-8.

  14. Oğuzoğlu TÇ, Muz D, Timurkan MÖ, Maral N, Gurcan IS. Prevalences of feline coronavirus (FCoV), feline leukaemia virus (FeLV), feline immunodeficiency virus (FIV) and feline parvovirus (FPV) among domestic cats in Ankara, Turkey. Revue Méd Vét. 2013;164:511-6.

  15. Koç BT, Oğuzoğlu TÇ. The investigation of feline parvoviruses (FPVs) into two different phylogenetic lineages in Turkey. J App Biol Sci. 2016;10:4-6.

  16. Gür S; Avdatek K. Afyonkarahisar’da kedilerde feline panleukopenia virusunun serolojik olarak araştirilmasi. Kocatepe Vet Derg. 2016;9:165-70.

  17. Sambrook J, Russell DW. Molecular cloning: a laboratory manual. 3rd ed. Huntington, NY: Cold Spring Harbor Laboratory Press; 2001.

  18. Pereira CA, Monezi TA, Mehnert DU, D’Angelo M, Durigon EL. Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay. Vet Microbiol. 2000;75:127-33.

  19. Battilani M, Balboni A, Ustulin M, Giunti M, Scagliarini A, Prosperi S. Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats. Vet Res. 2011;42:43.

  20. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol, 2013;30:2725-9.

  21. Campanella JJ, Bitincka L, Smalley J. MatGAT: an application that generates similarity/ identity matrices using protein or DNA sequences. BMC Bioinformatics. 2003;4:29.

  22. Rambaut A, Lam TT, Max Carvalho L, Pybus OG. Exploring the temporal structure of heterochronous sequences using TempEst (formerly Path-O-Gen). Virus Evol. 2016;2(9):vew007.

  23. Wang J, Cheng S, Yi L, Cheng Y, Yang S, Xu H, et al. Evidence for natural recombination between mink enteritis virus and canine parvovirus. Virol J. 2012;9:252.

  24. Battilani M, Ciulli S, Tisato E, Prosperi S, Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy, Virus Res. 2002;83:149-57.

  25. Carmichael LE, Schlafer DH, Hashimoto A. Minute virus of canines (MVC, canine parvovirus type-1): pathogenicity for pups and seroprevalence estimate. J Vet Diagn Invest 1994;6:165-74.

  26. Gordon JC, Angrick EJ. Canine parvovirus: environmental effects on infectivity. Am J Vet Res. 1986;47:1464-7.

  27. Chalmers WS, Truyen U, Greenwood NM, Baxendale W. Efficacy of feline panleucopenia vaccine to prevent infection with an isolate of CPV2b obtained from a cat. Vet Microbiol. 1999;69:41-5.

  28. Ikeda Y, Mochizuki M, Naito R, Nakamura K, Miyazawa T, Mikami T, et al. Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virology. 2000;278:13-9.

  29. Nakamura K, Sakamoto M, Ikeda Y, Sato E, Kawakami K, Miyazawa T, et al. Pathogenic potential of canine parvovirus types 2a and 2c in domestic cats. Clin Diagn Lab Immunol. 2001;8:663-8.

  30. Christensen J, Tattersall P. Parvovirus initiator protein NS1 and RPA coordinate replication fork progression in a reconstituted DNA replication system. J Virol. 2002;76:6518-31.

  31. Truyen U, Parrish CR. Feline panleukopenia virus: its interesting evolution and current problems in immunoprophylaxis against a serious pathogen. Vet Microbiol. 2013;165:29-32.




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