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2019, Number 2

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Vet Mex 2019; 6 (2)

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

Akkose M, Cebi SC, Kirmit A, Korkmaz O, Onur YM, Gocen M, Kuru M, Akçay E

Language: English/Spanish
References: 47
Page: 1-11
PDF size: 141.06 Kb.


ABSTRACT

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations were assessed in Holstein heifers 28 days post-insemination, following estrus synchronization with either a double prostaglandin (PG) injection or the ovsynch protocol. Animals between 15 and 20 months of age, with a 3-3.5 BCS, were randomly assigned to three groups: Heifers in the control group (n = 70) were monitored for estrus behaviour for 21 days and inseminated when detected in standing heat; Estrus of heifers in the Ovsynch group (n = 70)) was synchronized following the Ovsynch protocol, and timed artificial insemination was performed 16 h after the completion of treatment; Animals in the PG group (n = 70) received two prostaglandin-F2α injections 11 days apart, were monitored for estrus behaviour for 2-5 days following the last injection, and inseminated when in heat. Serum PAGs and progesterone concentrations were measured in pregnant heifers (control group n = 25; ovsynch group n = 22; PG group n = 21) on day 28 after artificial insemination (AI). Mean serum PAGs concentrations (OD: optical density) were similar between groups (control = 3.37 ± 0.148, Ovsynch = 3.21 ± 0.136, and PG = 3.34 ± 0.143; p › 0.05). However, serum progesterone concentrations were found to be lower in the ovsynch group (4.70 ± 0.17 ng/mL) when compared to the PG (5.34 ± 0.13 ng/mL) or the control (5.37 ± 0.08 ng/mL) groups (p ‹ 0.001). There was no correlation between serum PAGs and progesterone levels at day 28 post-insemination, or between early pregnancy PAGs concentrations and fetal death rates. Further research, including multiple sampling time points throughout gestation, after estrus synchronization protocols is warranted, to determine if there are associated temporal changes in PAGs and progesterone profiles that could impact reproductive parameters in dairy heifers.


REFERENCES

  1. Hughes AL, Green JA, Piontkivska H, Roberts RM. Aspartic proteinase phylogeny and the origin of pregnancy-associated glycoproteins. Mol Biol Evol. 2003;20(11):1940-5.

  2. Butler JE, Hamilton WC, Sasser RG, Ruder CA, Hass GM, Williams RJ. Detection and partial characterization of two bovine pregnancy-specific proteins. Biol Reprod. 1982;26(5):925-33.

  3. Mialon MM, Camous S, Renand G, Martal J. Menissier F. Peripheral concentrations of a 60-kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle. Reprod Nutr Dev. 1993;33(3):269-82.

  4. Atkinson YH, Gogolin-Ewens KJ. Hounsel EF, Davies MJ, Brandon MR, Seamark RF. Characterization of placentation-specific binucleate cell glycoproteins possessing a novel carbohydrate. J Biol Chem. 1993;268(35):26679-85.

  5. Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod. 1992;46(1):83-92.

  6. Sousa NM, Ayad A, Beckers JF, Gajewski Z. Pregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants. J Physiol Pharmacol. 2006;57 Suppl 8:153-71.

  7. Cain AJ, Christiansen D. Biochemical pregnancy diagnosis. In: Hopper RM, editor. Bovine reproduction. 1st ed. Pondicherry, India: Wiley Blackwell; 2015. p. 320-5.

  8. Egen TE, Ealy AD, Landon DA, Roberts RM, Green JA. Autoimmunization of ewes against pregnancy-associated glycoproteins does not interfere with the establishment and maintenance of pregnancy. Animal. 2009;3(6):850-7.

  9. Balhara AK, Gupta M, Singh S, Mohanty AK, Singh I. Early pregnancy diagnosis in bovines: current status and future directions. ScientificWorldJournal. 2013;2013:1-10.

  10. Akköse M, Cebi-Sen C. Sütçü sı􀍪ırlarda erken gebelik tanısı. Hay Uret. 2019. In press.

  11. Pohler KG, Gatea AO, Peres RFG, Pereira MHC, Vasconcelos JLM. Application of pregnancy associated glycoproteins (PAGs) to improve reproductive efficiency in cattle. Clinical Theriogenology. 2015;7(3):215-21.

  12. Wallace RM, Pohler KG, Smith MF, Green JA. Placental PAGs: Gene origins, expression patterns, and use as markers of pregnancy. Reproduction. 2015;149(3):115-26.

  13. Fricke PM, Ricci A, Giordano JO, Carvalho PD. Methods for and implementation of pregnancy diagnosis in dairy cows. Vet Clin North Am Food Anim Pract. 2016;32(1):165-80.

  14. Serrano B, López-Gatius F, Santolaria P, Almería S, García-Ispierto I, Bech-Sabat G, et al. Factors affecting plasma pregnancy-associated glycoprotein 1 concentrations throughout gestation in high-producing dairy cows. Reprod Dom Anim. 2009;44(4):600-5.

  15. López-Gatius F, Garbayo JM, Santolaria P, Yaniz J, Ayad A, deSousa NM, et al. Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Domest Anim Endocrinol. 2007;32(1):29-42.

  16. Ricci A, Carvalho PD, Amundson MC, Fourdraine RH, Vincenti L, Fricke PM. Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis. J Dairy Sci. 2015;98(4):2502-14.

  17. Kasimanickam R. Pharmacological intervention of estrus cycles. In: Hopper RM, editor. Bovine reproduction. 1st ed. Pondicherry, India: Wiley Blackwell; 2015.p. 304-13.

  18. Colazo MG, Mapletoft RJ. A review of current timed-AI (TAI) programs for beef and dairy cattle. Can Vet J. 2014;55(8):772-80.

  19. Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using PGF2alpha and GnRH. Theriogenology. 1995;44(7):915-23.

  20. Rabiee AR, Lean IJ, Stevenson MA. Efficacy of ovsynch program on reproductive performance in dairy cattle. A meta analysis. J Dairy Sci. 2005;88(8):2754-70.

  21. Bisinotto RS, Ribeiro ES, Santos JEP. Synchronisation of ovulation for management of reproduction in dairy cows. Animal. 2014;8(1):151-9.

  22. Vasconcelos JLM, Sartori R, Oliveira HN, Guenther JG, Wiltbank MC. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology. 2001;56(2):307-14.

  23. Perry GA, Smith MF, Lucy MC, Green JA, Parks TE, MacNeil MD, et al. Relationship between follicle size at insemination and pregnancy success. PNAS. 2005;102(14):5268–73.

  24. Vasconcelos JLM, Silcox RW, Pursley JR, Wiltbank MC. Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology. 1999;52(6):1067-78.

  25. Moreira F, de la Sota RL, Diaz T, Thatcher WW. Effect of day of the estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. J Anim Sci. 2000;78(6):1568-76.

  26. Wiltbank MC, Souza AH, Giordano JO, Nascimento AB, Vasconcelos JM, Pereira MHC, et al. Positive and negative effects of progesterone during timed AI protocols in lactating dairy cattle. Anim Reprod. 2012;9(3):231-41.

  27. Del Vecchio RP, Sutherland WD, Sasser RG. Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2 alpha. J Reprod Fertil. 1996;107(1):131-6.

  28. Weems YS, Lammoglia MA, Vera-Avila HR, Randel RD, King C, Sasser RG, et al. Effect of luteinizing hormone (LH), PGE2, 8-EPI-PGE1, 8-EPI-PGE2, trichosanthin, and pregnancy specific protein B (PSPB) on secretion of progesterone in vitro by corpora lutea (CL) from nonpregnant and pregnant cows. Prostaglandins Other Lipid Mediat. 1998;55(1):27-42.

  29. Ayad A, Sousa NM, Sulon J, Hornick JL, Watts J, Lopez-Gatius F, et al. Influence of progesterone concentrations on secretory functions of trophoblast and pituitary during the first trimester of pregnancy in dairy cattle. Theriogenology. 2007;67(9):1503-11.

  30. Barbato O, Merlo M, Celi P, Sousa NM, Guarneri L, Beckers JF, et al. Relationship between plasma progesterone and pregnancy-associated glycoprotein concentrations during early pregnancy in dairy cows. Vet J. 2013;195(3):385-7.

  31. Roberts JN, May KJ, Veiga-Lopez A. Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenology. 2017;89:271-9.

  32. Tandiya U, Nagar V, Yadav VP, Ali I, Gupta M, Dangi SS, et al. Temporal changes in pregnancy-associated glycoproteins across different stages of gestation in the Barbari goat. Anim Reprod Sci. 2013;142(3-4):141-8.

  33. Nagappan M, McGrath M, Schenkel R. Inventors. Methods for early detection of pregnancy in cows. Monsanto Technology LLC, assignee. 2009; US Pat. No. 7,604,950 B2 22.

  34. Kaya MS, Köse M, Bozkaya F, Mutlu H, Uçar EH, Atlı MO. Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows. Turk J Vet Anim Sci. 2016;40(6):1-6.

  35. Colazo MG, Gordon MB, Rajamahendran R, Mapletoft RJ, Ambrose DJ. Pregnancy rates to timed-AI in dairy cows treated with gonadotropin-releasing hormone or porcine luteinizing hormone. Theriogenology. 2009;72(2):262-70.

  36. Gumen A, Guenther JN, Wiltbank MC. Follicular size and response to ovsynch versus detection of estrus in anovular and ovular lactating dairy cows. J Dairy Sci. 2002;86(10):3184-94.

  37. Stevenson JS, Kobayashi Y, Thompson KE. Reproductive performance of dairy cows in various programmed breeding systems including OvSynch and combinations of gonadotropin-releasing hormone and prostaglandin F2a. J Dairy Sci. 1999;82(3):506-15.

  38. Tenhagen BA, Drillich M, Heuwieser W. Analysis of cow factors influencing conception rates after two timed breeding protocols. Theriogenology. 2001;56(5):831-8.

  39. Bartolome LA, Archbald LF. Reproductive management in dairy cows. In: Risco CA, Melendez P, editors. Dairy production medicine. 1st ed. London: Wiley Blackwell; 2011. p. 73-80.

  40. Taverne MA, Noakes DE. Pregnancy and its diagnosis. In: Noakes DE, Parkinson DJ, England GCW, editors. Veterinary reproduction and obstetrics. 9th ed. London: Saunders Elsevier; 2009. p. 76-122.

  41. Lobago F, Bekana M, Gustafsson H, Beckers JF, Yohannes G, Aster Y, et al. Serum profiles of pregnancy—associated glycoprotein, oestrone sulphate and progesterone during gestation and some factors influencing the profiles in Ethiopian Borana and crossbred cattle. Reprod Domest Anim. 2009;44(4):685-92.

  42. Silva E, Sterry RA, Kolb D, Mathialaghan N, McGrath MF, Ballam JM, et al. Accuracy of a pregnancy-associated glycoprotein ELISA to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial insemination. J Dairy Sci. 2007;90(10):4612-22.

  43. López-Gatius F, Hunter RH, Garbayo JM, Santolaria P, Yániz J, Serrano B, et al. Plasma concentrations of pregnancy-associated glycoprotein-1 (PAG-1) in high producing dairy cows suffering early fetal loss during the warm season. Theriogenology. 2007;67(8):1324–30.

  44. Pohler KG, Geary TW, Johnson CL, Atkins JA, Jinks EM, Busch DC, et al. Circulating bovine pregnancy associated glycoproteins are associated with late embryonic/ fetal survival but not ovulatory follicle size in suckled beef cows. J Anim Sci. 2013;91(9):4158–67.

  45. Pohler KG, Pereira MH, Lopes FR, Lawrence JC, Keisler DH, Smith MF, et al. Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds. J Dairy Sci. 2016;9(2):1584-94.

  46. Pohler KG, Peres RF, Green JA, Graff H, Martins T, Vasconcelos JL, et al. Use of bovine pregnancy-associated glycoproteins to predict late embryonic mortality in postpartum Nelore beef cows. Theriogenology. 2016;85(9):1652-9.

  47. Breukelman SP, Perényi Z, Taverne MA, Jonker H, van der Weijden GC, Vos PL, de Ruigh L, et al. Characterisation of pregnancy losses after embryo transfer by measuring plasma progesterone and bovine pregnancy-associated glycoprotein- 1 concentrations. Vet J. 2012;194(1):71-6.




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