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2019, Number 3

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Revista Habanera de Ciencias Médicas 2019; 18 (3)

First detection of Candidatus Rickettsia colombianensi in the State of Meta, Colombia

Sánchez Lerma, Liliana; Contreras Cogollo, Verónica; Mattar Velilla, Salim; Rodríguez González, Islay; Molina Atehortua, Andrés David; Castro Peñuela, Daniel Felipe

Language: Spanish
References: 24
Page: 487-499
PDF size: 733.09 Kb.


ABSTRACT

Introduction: Rickettsia is a genus of Gram-negative obligate intracellular bacteria that cause several epidemics around the world and are transmitted mainly by ticks, fleas, lice and mites. Rickettsiosis is a re-emergent infectious disease without epidemiological surveillance in Colombia. Until few years ago, Rickettsia rickettsi was the only tick-borne Rickettsioses in America; however, new species are being described, and although their pathogenicity has not been confirmed, they should be considered as potential pathogens.
Objective: The aim of this study was to determine the presence of rickettsial agents in ticks in the state of Meta, Colombia.
Material and Methods: A descriptive cross-sectional study was conducted; ticks were collected and, after their taxonomic identification, gltA, ompA and ompB genes were amplified and sequenced.
Results: A total of 169 pools were obtained to which PCR were carried out, of which 2 PCR were amplified for the gltA, ompA and ompB genes.
Conclusions: These results demonstrate the presence of Rickettsia spp in ticks in the State of Meta.


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