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ISSN 1561-3054 (Electronic)

2018, Number 3

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Rev Cubana Med Trop 2018; 70 (3)

Insecticide resistance in Aedes aegypti (Diptera: Culicidae) strains from three districts of the Central Pacific Region of Costa Rica

Calderón-Arguedas Ó, Vargas K, Troyo A

Language: Spanish
References: 23
Page: 1-9
PDF size: 237.47 Kb.


ABSTRACT

Introduction: Aedes aegypti is the vector of dengue, chikungunya, and Zika viruses in Costa Rica. The high incidence and the lack of vaccines make vector control, including chemical control, the only measure to prevent transmission. The repetitive use of insecticides may induce resistance.
Objective: To determine resistance and enzymatic detoxifying mechanisms to temephos and pyrethroids insecticides in strains of Ae. aegypti from three districts of the Central Pacific Region of Costa Rica.
Methods: Resistance to temephos, deltamethrin, and cypermethrin was determined in three strains of Barranca, Jacó, and Quepos by larval bioassays. In each test, the lethal concentration 50 % (LC50) and a factor of resistance 50 % (FR50) were calculated, using the Rockefeller strain as control. When resistance was observed, the bioassays were repeated using piperonyl butoxide, S,S,S, tributylphosphorotritioate, and ethacrynic acid, that inhibit monoxygenases, esterases, and glutathione S- transfererase, respectively.
Results: None of the strains were resistant to temephos. Resistance to deltamethrin (FR50= 7.38 and FR50= 28.23, respectively) was determined in the strains from Barranca and Jacó, while resistance to cypermethrin was detected only in Jacó (FR50= 7.70). The Quepos strain was not resistant to any pyrethroid. Only the Barranca strain showed a decrease in the resistance to deltamethrin when piperonyl butoxide was used, linking the resistance to monooxygenase enzymes (FR50: 10.10). For the other cases, none of the synergists decreased the resistance.
Conclusions: Larvae of Ae. aegypti from the localities evaluated were not resistant to temephos. With respect to pyrethroids, results show an emergence of resistance that may not be mediated by enzymatic detoxification.


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