Bracho CA, Jaramillo ACJ, Martínez MJJ, Montaño HJA, Olguín BA

Language: English/Spanish
References: 34
Page: 161-163
PDF size: 330.99 Kb.

ABSTRACT

The present study compares the specificity (Sp) and sensibility (Se), as well as the positive (+PV) and negative predictive (–PV) values of immunoperoxidase (IP) and immunofluorescence (IF) tests for the diagnosis of abortions caused by IBR, as well as their concordance with serum neutralization in the dams of the fetuses, compared against a standard test of viral isolation.(VI) Forty-nine aborted fetuses, obtained over a one-year period, were evaluated. Liver, spleen, kidney and lung samples were collected from each fetus. Serum from the dams was collected on three occasions, one month apart, starting immediately after the abortion. Samples were taken from animals in “Agricultural and Industrial Complex of Tizayuca (CAIT)” Tizayuca, Hidalgo, and the diagnostic technique was carried out in “National Center of Diagnostic Services in Animal Health (CENASA)” as prescribed by the World Organization for Animal Health (OIE). A total of 29 viral isolations of IBR were obtained. The immunoperoxidase (IP) and immunofluorescence (IF) techniques had a sensitivity of 96.7% and 24.1%, respectively, and a specificity of 20% and 70%, respectively. The positive predictive values for these were 64.4% and 53.8%, respectively, while the negative predictive values were 80% and 38.9%, respectively. The concordance for both tests was bad. Of the organs sampled, the liver and kidney were the best for IF and IP diagnosis of IBR, respectively. Concordance for serum neutralization, immunofluorescence and immunoperoxidase in relation to viral isolation was not significant (P › 0.05).

REFERENCES

1. Barr BC, BonDurant RH. Viral Diseases of the fetus. In: Youngquist RS, editor. Bovine theriogenology. Philadelphia: W:B. Sauders Company, 2000:373-381.

2. Van der Poel WH, Kramps JQ, Brand A, Van Oirschot JT. Persistence of bovine herpesvirus-1-specific antibodies in cattle after intranasal vaccination with a live virus vaccine.Vet Rec 1995;137: 347-348.

3. Kramps JA, Perrin B. A European inter-laboratoty trial to evaluate the reability of serological of bovine herpesvirus 1 infections. Vet Microbiol 1996; 53:156-161.

4. Organización Panamericana de la Salud-Organización Mundial de la Salud. Programa de adiestramiento en salud animal para América Latina, Cuarentena Animal. México (DF): OPS, 1986.

5. Aguilar AS. El virus de la rinotraqueítis infecciosa bovina (Bovid herpesvirus 1): propiedades y vacunación. Cienc Vet 1987; 4:161-202.

6. Barajas J, Riemann H, Franti C. Aplication of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of Mexico. Rev Sci Tech Off Int Epiz 1993; 12: 717-732.

7. Howard J. Current Veterinary Theraphy 3: Food Animal Practice. Philadelphia: Saunders,1993.

8. Pastoret PP, Thiry E, Brochier B, Derboven G. Bovid herpes virus infection of cattle: phathogenesis, latency, consequences of latency. Ann Rech Vet 1992;13: 221-235.

9. Wohlgemuth K, Domínguez J, López L. El complejo respiratorio bovino: etiología viral. Memorias del XIV Congress on Veterinary Sciences. PANVET. 1994 octubre 9-15; Acapulco (Guerrero) México (DF): Asociación Panamericana de Ciencias Veterinarias, 1994: 430-432.

10. Oficina Internacional de Epizootias. Manual of Standards for Diagnostic Tests and Vaccines. Organismo Internacional de Epizootias 1995; 322-328.

11. Aguilar S. Recomendaciones para la prevención contra la rinotraqueítis infecciosa bovina (Bovis herpesvirus 1, BHV-1). Memorias del V Curso Internacional de Reproducción Bovina; 1993 mayo 11-13; Distrito Federal (México) México (DF): Academia de la investigación en biología de la reproducción. 1993: 253:260.

12. Smith DR. Veterinary clinical epidemiology: A problem oriented approach. Buenos Aires- Argentina: University of Illinois E.U.A, 1990.

13. Brock K, Potgieter N. Rapid fluorescence detection of in situ hybridization with biotinylated bovine herpesvirus-1 DNA probes. J Vet Diagn Invest 1989; 1:34-38.

14. Bratanich A, Sardi S, Smitsaar E, Estevez M, Schudel A. Comparison of three serological techniques for the diagnosis of bovine herpesvirus type 1: serumneutralization, enzyme-linked immunosorbent assay and indirect immunofluorescence. Rev Argent. Microbiol 1990; 22: 192-198.

15. Delgado I, Barrera M, Tuero C, Rodríguez N. Comparación de tres métodos de detección de antígeno para el diagnóstico de herpes bovino tipo 1. Salud Animal 1992; 14: 143-148.

16. Babiuk LA, Van Drunen S, Tikoo SK. Inmunology of bovine herpesvirus 1 infection. Vet Microbiol 1996; 53: 31-42.

17. Smith GH, Collins KJ, Carman J. Use of immunoperoxidase test for the detection of bovine herpesvirus-1 in aborted fetal tissue. J Vet Diagn Invest 1989;1:39-44.

18. Gimeno E, Belak K, Massone A, Echeverria M, Ibargoryen G. Técnicas inmunohistoquímicas en patología veterinaria: Aspectos Teóricos y Prácticos. Vet Argent 1988; 5: 332-338.

19. Oficina Internacional de Epizootias. Manual of recommended diagnostic techniques and requirements for biological products for list A an B diseases. 1990; Vol. II.

20. Organización de las Naciones Unidas para la Agricultura y la Alimentación. Oficina regional para América Latina y el Caribe. Red de Cooperación Técnica entre Laboratorios de Investigación y diagnóstico veterinario. Manual de técnicas para diagnóstico virológico. FAO Centro de investigaciones de ciencias veterinarias INTA, Argentina, 1987.

21. Aluja SA. Necropsias en animales domésticos. México DF: Continental, 1985.

22. Perrin B, Calvo T, Cordioli P, Edwars S, Eloit M, Guerin B, et al. Selection of european union standard reference sera for use in the serological diagnosis of infectious bovine rhinotracheitis. Rev Sci Tech Off Int Epiz 1994;13:947-960.

23. Galen R S. Use of predictive value theory in clinical immunology. Manual of clinical laboratory immunology. 3a ed. Washington DC : A Soc Micro 1986.

24. Morton A, Hebel C. Bioestadística y Epidemiología. 3a ed. México DF: Interamericana, Mc Graw-Hill, 1993.

25. Theodoris A. Studies on bovine herpesviruses. Part 1. Isolation and characterization of viruses isolated from the genital tract of cattle. Onderstepoort J Vet Res 1985; 52:239-254.

26. Kelling CL, Schipper IA, Haugse CN. Antibody response in calves administration of attenuated infectious bovine rinothracheitis (IBR) vaccine. Can J Comp Med 1973; 37: 309-312.

27. Posposil Z, Krejci J, Machatkova M. Development of a disease control programme based on the use of an inactivated vaccine againts infectious bovine rhinotracheitis. Vet Microbiol 1996; 53: 199-206.

28. Reed DE, Bicknell EJ, Larson CA, Knudtson WU, Kikbride CA. Infectious bovine rhinotracheitis virus-induced abortion: rapid diagnosis by fluorescent antibody technique. J Vet Res 1971, 32: 1423-1426.

29. Van Drunen S, Van den Hurk, Tikoo SK, Babiuk LA, Donkersgoed JV. Protective immunity in cattle following vaccination with conventional and marker bovine herpesvirus-1 (BHV1)vaccines. Vaccine 1997; 15: 36-44.

30. Kaashhoek JM, Rijsewijk JT. Persistence of antibodies against bovine herpesvirus 1 and virus reactivation two to three years after infection. Vet Microbiol 1996;53: 103-110.

31. Baker JC, Steven RR, Walter DR. Transmission of a vaccinal strain of infectious bovine rhinotracheitis virus from intranasally vaccinated steers commingled with nonvaccinated steers. Am J Vet Res 1989; 50: 814-816.

32. Mars MH, Bruschke CJ, Van Oirschot JT. Airborne transmission of BHV1, BRSV, and BVDV among cattle is possible under experimental conditions. Vet Microbiol 1999; 66: 197-207.

33. Moraga BL, Berrios PE, Zurita LA, Celedón M, Guajardo U, Ortiga A. Rinotraqueítis Infecciosa Bovina III. Infeccción experimental en vaquillas gestantes. Avances en Cienc Vet 1990;5:129-135.

34. Biuk RN, Cvetnic S, Madic J, Rudan D. Prevalence of antibodies to IBR and BVD viruses in diary cows with reproductive disorders. Theriogenology 1998; 875-881.