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ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2018, Number 2

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Biotecnol Apl 2018; 35 (2)

Evaluation of the bioformulate and raw filtrate of Purpureocillium sp. strain Udea0106 on plant pathogenic nematodes in chrysanthemum (Dendranthema grandiflora) crops

Sánchez JY, Cardona NL

Language: English
References: 38
Page: 2221-2227
PDF size: 457.12 Kb.


ABSTRACT

Plant-parasitic nematodes are the main agricultural pest affecting the floricultural industry of Colombia. Taking into account previous research where strain UdeA0106 of the Purpureocillium sp. fungus was shown to exhibit a high nematicidal capacity, the present work evaluated different concentrations of a raw filtrate from this biocontrol agent and performed a field experiment with a bioformulation thereof. The variables evaluated in both cases were the genus and numbers of nematodes in soil and the gall index. Additionally, the field trial evaluated productivity variables such as the number of flower buds, plant weight and height and stem width. All the evaluations were repeated twice, and the existence of statistically significant inter-treatment differences was detected via ANOVA or Kruskal-Wallis tests, using 0.05 as cut off for statistical significance, as well as multiple comparison tests such as Tukey’s and kruskalmc, as implemented in R®. The raw filtrate produced a statistically significant decrease in the number of juvenile-stage Meloidogyne spp. and Paratylenchus spp. nematodes in soil, although it failed to reduce root infestation indexes. The bioformulation from strain UdeA0106 also produced statistically significant decreases in the number of nematodes when compared to control treatments. In the case of the nodulation variable, both the filtrate and the bioformulation of UdeA0106 yielded lower numbers and indexes compared to the chemical products evaluated. For the “weight” productivity variable, the bioformulation field experiment showed differences between treatments with 4.6 g, a desirable aspect in the production of flowers.


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