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2019, Number S1

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Gac Med Mex 2019; 155 (S1)

Detección y caracterización molecular de virus respiratorios causantes de infección respiratoria aguda en población adulta

Díaz-Chiguer DL, Tirado-Mendoza R, Márquez-Navarro A, Ambrosio-Hernández JR, Ruiz-Fraga I, Aguilar-Vargas RE, Lira-Martínez JM, López-Valdés JC

Language: Spanish
References: 28
Page: 16-21
PDF size: 141.65 Kb.


ABSTRACT

Introduction: Acute respiratory infections are one of the main causes of morbidity and mortality in older adults and patients with chronic diseases. Among the responsible etiological agents are human respiratory viruses, such as: respiratory syncytial virus, parainfluenza virus and metapneumovirus. Objective: To carry out a differential diagnostic study of respiratory viruses circulating and co-circulating in an adult population. Methods: A pilot study was conducted in patients older than 18 years, who presented signs and symptoms suggestive of acute respiratory infection and whose clinical picture did not exceed 15 days of evolution; end-point polymerase chain reaction assays were performed with the use of specific oligonucleotides for molecular diagnosis. Results: 72 specimens of patients with an age of 51.33 ± 19.33 years, with a predominance of females (4.5:1); original inhabitants of Mexico City; only 22 were positive for respiratory viruses, being mostly metapneumovirus infections. Conclusions: The knowledge of the circulating viral strains in the population will allow to determine changes that can declare an epidemiological alert leading to the best decision making for the benefit of the patients.


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  5. Colosia AD, Yang J, Hillson E, Mauskopf J, Copley-Merriman C, Shinde V, et al. The epidemiology of medically attended respiratory syncytial virus in older adults in the United States: A systematic review. PLoS One. 2017;12 e0182321.

  6. Volling C, Hassan K, Mazzulli T, Green K, Al-Den A, Hunter P, et al. Respiratory syncytial virus infection-associated hospitalization in adults: a retrospective cohort study. BMC Infect Dis. 2014;14:665.

  7. Mackay IM, Jacob KC, Woolhouse D, Waller K, Syrmis MW, Whiley DM, et al. Molecular assays for detection of human metapneumovirus. J Clin Microbiol. 2003;41:100-5.

  8. Fields B, Knipe DM, Howley PM. Virology. Philadelphia: Wolters Kluwer/ Lippincott Williams & Wilkins; 2007.

  9. Romero Feregrino R, Romero Feregrino R, Magaña IM, Romero Cabello R, González Saldaña NJ. Diagnóstico de Metapneumovirus humano. Revista de Enfermedades Infecciosas en Pediatría. 2013;26:256-69.

  10. Visseaux B, Burdet C, Voiriot G, Lescure FX, Chougar T, Brugière O, et al. Prevalence of respiratory viruses among adults, by season, age, respiratory tract region and type of medical unit in Paris, France, from 2011 to 2016. 2017;12:e0180888.

  11. Instituto Mexicano del Seguro Social. Guía de práctica clínica de diagnóstico y tratamiento de bronquiolitis aguda en niñas/niños y en el Primer Nivel de Atención. México; 2015.

  12. Dayakar S, Pillai HR, Thulasi VP, Nair RR. Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens. 2016;27:375-81.

  13. Stockton J, Stephenson I, Fleming D, Zambon MJ. Human metapneumovirus as a cause of community-acquired respiratory illness. 2002;8:897.

  14. Martinello RA, Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Human metapneumovirus and exacerbations of chronic obstructive pulmonary disease. 2006;53:248-54.

  15. Pio A, Leowski J, Luelmo F. Programa de la Organización Mundial de la Salud de infecciones respiratorias agudas en la infancia. Bol Of Sanit Panam. 1984;96(4):283-93.

  16. Secretaria de Salud. [internet] Anuario Estadístico 2016. Epidemiología. Disponible en www.epidemiologia.salud.gob.mx/anuario/ html/anuarios.

  17. Wylie KM. The virome of the human respiratory tract. Clin Chest Med. 2017;38:11-9.

  18. Ogra PL. Respiratory syncytial virus: the virus, the disease and the immune response. Paediatr Respir Rev. 2004;5(Suppl A):S119-26.

  19. Colosia AD, Yang J, Hillson E, Mauskopf J, Copley-Merriman C, Shinde V, et al. The epidemiology of medically attended respiratory syncytial virus in older adults in the United States: A systematic review. PLoS One. 2017;12 e0182321.

  20. Volling C, Hassan K, Mazzulli T, Green K, Al-Den A, Hunter P, et al. Respiratory syncytial virus infection-associated hospitalization in adults: a retrospective cohort study. BMC Infect Dis. 2014;14:665.

  21. Mackay IM, Jacob KC, Woolhouse D, Waller K, Syrmis MW, Whiley DM, et al. Molecular assays for detection of human metapneumovirus. J Clin Microbiol. 2003;41:100-5.

  22. Fields B, Knipe DM, Howley PM. Virology. Philadelphia: Wolters Kluwer/ Lippincott Williams & Wilkins; 2007.

  23. Romero Feregrino R, Romero Feregrino R, Magaña IM, Romero Cabello R, González Saldaña NJ. Diagnóstico de Metapneumovirus humano. Revista de Enfermedades Infecciosas en Pediatría. 2013;26:256-69.

  24. Visseaux B, Burdet C, Voiriot G, Lescure FX, Chougar T, Brugière O, et al. Prevalence of respiratory viruses among adults, by season, age, respiratory tract region and type of medical unit in Paris, France, from 2011 to 2016. 2017;12:e0180888.

  25. Instituto Mexicano del Seguro Social. Guía de práctica clínica de diagnóstico y tratamiento de bronquiolitis aguda en niñas/niños y en el Primer Nivel de Atención. México; 2015.

  26. Dayakar S, Pillai HR, Thulasi VP, Nair RR. Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens. 2016;27:375-81.

  27. Stockton J, Stephenson I, Fleming D, Zambon MJ. Human metapneumovirus as a cause of community-acquired respiratory illness. 2002;8:897.

  28. Martinello RA, Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Human metapneumovirus and exacerbations of chronic obstructive pulmonary disease. 2006;53:248-54.




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