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2018, Number 1

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Rev Cubana Invest Bioméd 2018; 37 (1)

Performance of blood culture HemoCen aerobic neonatal with clinical samples in hospitals in Havana, Cuba

López RY, Zhurbenko R, Rodríguez MC, Someillan ID, Ortega SA, Ortiz RC, Álvarez PAB, Pérez AJ

Language: Spanish
References: 20
Page: 65-74
PDF size: 224.42 Kb.


ABSTRACT

Introduction: Neonates have high probability for developed bacteremia due to the immaturity of their immune system. The conventional method used for the diagnosis of bateremia is the inoculation of blood samples inspecial culture media for hemoculture. The ready to use HemoCen aerobic neonatal culture medium was designed in the National Center of Bioproducts (BioCen, Cuba).
Objectives: To evaluate the performance of HemoCen aerobic neonatal in clinical samples from three hospitals. To demonstrate that this medium fit to the purpose for which it was designed.
Methods: 96 blood samples from neonates were evaluated inoculating them into hemoculture bottles. The identification of microorganisms was executed for all positive samples. The diagnostic capacity of the culture medium was determined by the following variables: positivity, type of bacteria isolated, sensitivity, specificity and diagnostics accuracy and also, predictive value of the positive and negative results.
Results: Among all the samples tested, 11 where considered positives (11,5 % positivity), detecting the presence of 8 species of microorganisms:Staphylococcus saprophyticus, Enterobacter cloacae,Escherichia coli, Enterobacter aerogenes,Staphylococcus coagulasenegative, Klebsiella ozaenae, Staphylococcus aureus and Candida spp. HemoCen aerobic neonatal showed high values of diagnostic indicators.
Conclusions: HemoCen aerobic neonatal successfully detected the presence of several of the main pathogens that cause bacteremia and fungemias in blood samples of newborns, concluding that it meets the purpose for which it was designed.


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