Dorta-Contreras AJ, González-Losada C, Sánchez MC

Language: Spanish
References: 8
Page: 826-836
PDF size: 895.73 Kb.

ABSTRACT

Introduction: Single radial immunodiffusion assay is a technique with immunological base, which is reliable because of its specificity in the quantification of main immunoglobulins, although it is also used for other proteins. Commercial immunodiffusion plates are offered with a determined number of holes where the biological samples containing protein to be quantified are placed.
Objective: To evaluate the sensitivity and specificity of the modification implemented to optimize the usage of single radial immunodiffusion plates from Siemens by increasing the number of samples in the plates.
Materials and Methods: An innovating procedure that allows to optimize the non-used biologically active area and use it in the quantification of other samples is presented. A parallel quantification of control samples from traditional holes and the other ones opened in available spaces was performed in order to quantify IgG and albumin in serum and in cerebrospinal fluid.
Results: Sensitivity was not affected significantly between the normal plates and the usage of the new procedure. Regarding specificity, there are also no significant differences except in the plates used to quantify serum albumin; so, it is recommended to dilute serum samples before the application. In case of NOR and LC Partigens®, this proposed modification duplicates the number of samples to be quantified in each plate, but otherwise, it could be applied in other commercial immunoplates.
Conclusions: This innovation allows to make an optimal usage of immunodiffusion plates with the consequent saving of import materials, which can be easily applied in all the laboratories of the country.

REFERENCES

1. Reiber H. Cerebrospinal fluid data compilation and knowledge-based interpretation of bacterial, viral, parasitic, oncological, chronic inflammatory and demyelinating diseases. Diagnostic patterns not to be missed in neurology and psychiatry. Arq Neuropsiquiatr. 2016; 74(4):337-350.

2. Wildemann B, Oschmann P, Reiber H. Laboratory diagnosis in Neurology. Stuttgart: Thieme; 2010.

3. Choi Y, Lee S, Kwon SY, Lee Y, Park YK, Ban SJ. Analysis of the proficiency of single radial immunodiffusion assays for quality control of influenza vaccines in Korea. Biologicals. 2017 Nov[consultado 13/09/2018]; 50:137-140. Disponible en: https://www.sciencedirect.com/science/article/ pii/S1045105617300969?via%3Dihub

4. Padrón-González AA, González-Losada C, Dorta Contreras A. Empleo del Reibergrama en manifestaciones neurológicas del dengue. Rev haban cienc méd. 2017[consultado: 20/12/2017]; 16(5):711-719. Disponible en: http://www.revhabanera.sld.cu/index.php/rhab /article/view/2004

5. Martini Robles L, Dorta Contreras AJ, editores. Angiostrongylus cantonensis. Emergencia en América. La Habana: Academia; 2016. 7-17.

6. Betancourt Loza M, Cordero Eiriz A, Peña Sánchez M, González García S, Lorigados Pedres L, González–Quevedo A. Cuantificación de la inmunoglobulina G y la albúmina en el líquido cefalorraquídeo mediante las modificaciones de las técnicas para otros fluidos biológicos. Rev Cubana Neurol Neurocir. [Internet]. 2016 [consultado 29/09/2018];6(1):9-16. Disponible en: http://www.medigraphic.com/pdfs/revcubneur o/cnn-2016/cnn161b.pdf

7. Reiber H. Knowledge-base for interpretation of cerebrospinal fluid data patterns. Essentials in neurology and psychiatry. Arq Neuropsiquiatr. 2016; 74(6): 501-512.

8. Reiber H. Polyspecific antibodies without persisting antigen in multiple sclerosis, neurolupus and Guillain-Barré syndrome: immune network connectivity in chronic diseases. Arq Neuropsiquiatr. 2017; 75(8):580- 588.