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2017, Number 4

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Enf Infec Microbiol 2017; 37 (4)

Contamination by Brucella sp. and lack of compliance in measures of basic sanitation in dairy production

Martínez RA, Ortega SJL, Cervantes FM, Urtiz EN, Hernández GSI, Alba RJJ

Language: Spanish
References: 18
Page: 128-132
PDF size: 215.64 Kb.


ABSTRACT

Brucellosis is considered a zoonosis caused by Brucella sp.; in the human is the accidental expression of disease in animals.
Objective. To identify contamination by Brucella sp. and lack of compliance in basic sanitation measures in dairy production.
Material and Methods. Observational, descriptive and cross-sectional study. Samples of dairy products destined for human consumption were made in the herds named A, B and C of Banco Nacional, Tlahualilo, Durango, where 300 samples of dairy products were analyzed. They were cultivated on tripticasein soy agar, MacConkey agar and Brucella agar; biochemical tests as well as immunoassay with polyvalent anti-Brucella serum were performed. Confirmation was made on MicroScan autoscan-4 Siemens ®.
Results. The genus Brucella sp. was isolated in 12% of the analyzed samples; in 102 (34%) it was not possible to determine the presence of Brucella due to the high concentration of contaminating flora, in 54% (162 samples) the main etiological agents isolates were: Klebsiella sp., Proteus sp. and Escherichia coli.
Conclusions. In addition to the isolation of Brucella sp. in the dairy samples, enterobacteria were also isolated, suggesting a high degree of contamination. It is essential to take measures in the hygienic management of dairy products. The implementation of the Basic sanitation strategies will allow the application of actions to prevent and control the risks present in the production of dairy products that are commercialized in the Comarca Lagunera.


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