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2018, Number 3

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Rev Educ Bioquimica 2018; 37 (3)

Estudio del transcriptoma mediante RNA-seq con énfasis en las especies vegetales no modelo

Rodríguez-Alonso G, Shishkova S

Language: Spanish
References: 68
Page: 75-88
PDF size: 720.74 Kb.


ABSTRACT

The advance of biology has largely relied on the detailed study of a small number of species collectively known as model organisms. These species were chosen as experimental models due to their peculiarities, which represent practical advantages for their study in laboratory conditions. However, the improvement of sequencing technologies allows nowadays the genome and transcriptome sequencing of virtually any organism without the need of a reference genome, and therefore, new species can be used now to study biological processes that were previously inaccessible from the study of classic model organisms. In this review, we present a general description of the main sequencing technologies that can be used for transcriptome sequencing (RNA-seq) and provide an overview of transcriptome assembly and analysis when no reference genome is available. Finally, some examples of transcriptome analysis applied to non-model organisms are provided.


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