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ISSN 0185-3252 (Print)
Revista de la Asociación Médica del Centro Médico ABC

2006, Number 1

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An Med Asoc Med Hosp ABC 2006; 51 (1)

Serum and intrafollicular expression of leptine concentration as a marker in the characteristics of ovocyte development

Cruz V, Corona C, Avendaño A, Barroso G

Language: Spanish
References: 25
Page: 5-10
PDF size: 96.13 Kb.


ABSTRACT

Objective: To determine the conditions of egg maturity and embryonic development serum and intrafollicular concentration of leptine. Material and methods: Prospective and observational study. A total of 30 cycles of controlled ovarian hyperstimulation of IVF/ICSI procedures were included, where concentration of leptine in serum and intrafollicular fluid was measured. This measure was made from individual follicles during the egg retreival where fertilization characteristics were evaluated. Results: During a controlled ovarian hyperstimulation cycle of 30 patients, divided in two groups of ages (‹ 35 y/o and › 35 y/o), there were no differences on base serum levels of FSH, LH and IMC by study design between groups. There exists a directly proportional relation between the high levels of serum estradiol and the quantity of fertilized ova in patients ‹ 35 y/o which respectively correlates with a lower concentration of leptine in day 3 of the menstrual cycle and day of application of hGC that demonstrated statistics significance (P‹ 0.05). Conclusions: Patients › 35 y/o underwent ovarian hyperstimulation cycles, showed higher concentrations of serum and intrafollicular leptine related to a low concentration of estradiol, where this condition of intrafollicular contraregulation is expressed by a lower number of fertilized ova and reduction on ovum quality, possibly requiring a higher amount of r-FSH for its stimulation.


REFERENCES

  1. Bützow T, Moilanen J, Lehtovirta M, Tuomi T, Hovatta O, Siegberg R, Nilson C, Apter D. Serum and follicular fluid leptin during in vitro fertilization: Relationship among leptin increase, body fat mass, and reduced ovarian response. J Clin Endocrinol Metab 1999; 84 (9): 3135-3139.

  2. Van Blerkom J, Antczak M, Schrader R. The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: Association with vascular endothelial growth factor levels and perifollicular blood flow characteristics. Hum Reprod 1997; 12 (12): 1047-1055.

  3. Van Blerkom J. Epigenetic influences on oocyte developmental competence: Perifollicular vascularity and intrafollicular oxygen. J assist Reprod Genet 1998; 15 (5): 226-234.

  4. Nargund G, Bourne T, Doyle P, Parsons J, Cheng W, Campbell S, Collins W. Associations between ultrasound indices of follicular blood flow oocyte recovery and preimplantation embryo quality. Hum Reprod 1996; 11 (1): 109-113.

  5. Huey S, Abuhamad A, Barroso G, Hsu MI, Kolm P, Mayer J, Oehninger S. Perifollicular blood flow doppler indices, but no follicular pO2, pCO2, or pH, predict oocyte developmental competence in vitro fertilization. Fertil Steril 1999; 72 (4): 707-712.

  6. Hartshorne GM. Preovulatory follicular fluid: Relationships to ovarian stimulation protocol, fertilization, and sperm penetration in vitro. Fertil Steril 1989; 52 (6): 998-1005.

  7. Chehab F, Lom M, Lu R. Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin. Nature Gene 1996; 12: 318-320.

  8. Cioffi J, Van Blerkom J, Antczak M et al. The expression of leptin and its receptors in pre-ovulatory human follicles. Mol Hum Reprod 1997; 2: 467-472.

  9. Zhang Y, Proenca R, Maffei M et al. Possitional cloning of the mouse obse gene and its human homologue. Nature 1994; 372: 425-432.

  10. Ogawa Y, Masuzaki H, Isse N et al. Molecular cloning of rat obese cDNA and augmented gen expression in generally obese Zucker fatty (fat/fat) rats. J Clin Investig 1995; 96: 87-94.

  11. Frunhbereck G, Jebb S, Prentice A. Leptin: Physiology and pathophysiology. Clin Physiol 1998; 18: 399-419.

  12. Cunningham M, Clifton D, Steiner R. Leptin´s actions on the reproductive axis; perspectives and mechanisms. Biol Reprod 1999; 60: 216-222.

  13. Licinio J, Negartilde A, Mantzoros C et al. Synchronicity of frequently sampled, 24-h concentrations of circulating leptin, luteinizing hormone, and estradiol in healthy women. Proc Natl Acad Sci USA 1998; 95: 2541-2546.

  14. Mannucci E, Ognibene A, Becorpi A et al. Relationship between leptin and estrogens in healthy women. Eur J Endocrinol 1998; 139: 198-201.

  15. Messinis I, Milingos S, Zikopoulos K et al. Leptin concentrations in the follicular phase of spontaneous cycles superovulated with follicle stimulating hormone. Hum Reprod 1998; 13: 1152-1156.

  16. Korbonits M, Trainer P, Litle J et al. Leptin levels do not change acutely with food administration in normal or obese subjects, but are negatively correlated with pituitary-adrenal activity. Clin Endocrinol 1997; 46: 751-757.

  17. Gonzalez R, Simon C, Caballero P, Norman R, Chardonnens D, Devoto L, Bischof P. Leptin and reproduction. Hum Reprod 2000; 6 (3): 290-300.

  18. Unkila-Kallio L, Andersson S, Koistinen H. Karonen S, Ylikorkala O, Tiitinen A. Leptin during assisted reproductive cycles: the effect of ovarian stimulation and of very early pregnancy. Hum Reprod 2001; 16 (4): 657-662.

  19. Barroso G, Barrionuevo M, Rao P, Grahan L, Danforth D, Huey S, Abuhamad A, Oehinger S. Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients. Fertil Steril 1999; 16: 1024-1026.

  20. Mantzoros CHS, Cramer DW, Liberman RF, Barbieri RL. Predictive value of serum and follicular fluid leptin concentrations during assisted reproductive cycles in normal women and in women with the polycystic ovarian syndrome. Hum Reprod 2000; 15: 539-544.

  21. Gurbuz B, Yalti S, Ficicioglu C, Tasdemir S. The relation of serum and follicular fluid leptin and ovarian steroid levels in response to induction of ovulation in vitro fertilization cycles. Eur J Obstet Gynecol Reprod Biol 2005; 118 (2): 214-218.

  22. Anifandis G, Koutselini E, Louridas K, Liakopoulos V, Leivaditis K, Mantzavinos T, Sioutopoulou D, Vamvakopoulos N. Estradiol and leptin as conditional prognostic IVF markers. Hum Reprod 2005; 129 (4): 531-534.

  23. Caprio M, Fabbrini E, Isidori AM, Aversa A, Fabbri A. Leptin in reproduction. Trends Endocrinol Metab 2001; 12: 65-72.

  24. Moschos S, Chan JL, Mantzoros CS. Leptin and reproduction: a review. Fertil Steril 2002 (77): 433-444.

  25. Karlsson C, Lindell K, Svensson E, Bergh C, Lind P, Billig H, Carlsson LMS, Carlsson B. Expression of functional leptin receptors in the human ovary. J Clin Endocrynol Metab 1997; 82: 4144-4148.




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