Martínez-Flores F, Sandoval H, Arce VE, García-Cavazos RJ, Jiménez-Orozco FA, Valdes-Flores M, Madinaveitia-Villanueva JA

Language: Spanish
References: 23
Page: 237-243
PDF size: 527.24 Kb.

ABSTRACT

Introduction: The early growth response protein (EGR-1) is a transcription factor involved in cell differentiation and proliferation, whose expression is regulated by its promoter in response to various physical, chemical and drug factors. Hereby, we describe some of the main effects of steroid drugs and EGF-1 on promoter activity, through a reporter system transduced by Ad Δegr-1-Luc7 in human primary fibroblasts (HPF). Methods: Human primary fibroblasts transduced with Ad Δegr-1-Luc7 were exposed to betamethasone, hydrocortisone, dexamethasone, testosterone, beta-estradiol, and EGF-1 during 1, 3 and 6 h. Reporter assay was quantified by luminometry. Results: The activity of the promoter in presence of betamethasone, hydrocortisone, dexamethasone, testosterone and beta-estradiol were similar to the basal activity of the promoter at 1, 3 and 6 h. The positive control showed an activity 17.8 folds higher (p ≤ 0.05) at 6 h. EGF-1 showed activity of 22.07 folds greater than cells without drug. Conclusion: The activity of the EGR-1 promoter in human fibroblasts is negatively regulated by steroid drugs and positively by the EGF-1.

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