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2018, Number 5

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Ann Hepatol 2018; 17 (5)

Fast Morphological Gallbladder Changes Triggered by a Hypercholesterolemic Diet

López-Reyes A, Clavijo-Cornejo D, Fernández-Torres J, Medina-Luna E, Estrada-Villaseñor EG, Gómez-Quiroz LE, Gutiérrez M, Granados J, Vargas-Alarcón G, Pineda C, García H, Morales-Garza LA, Gutiérrez-Ruiz MC, Martínez-Flores K

Language: English
References: 35
Page: 857-863
PDF size: 1333.28 Kb.


ABSTRACT

Introduction and aim. Obesity is a worldwide epidemic problem, described as a risk factor for hepatic diseases, such as non-alcoholic fatty liver disease and other pathologies related to development of cholesterol crystals and cholesterol gallbladder stones. It has been reported that cholesterol overload may cause hepatic damage; however, little is known about the effects of an acute hypercholesterolemic diet on the gallbladder. The aim of this manuscript was to evaluate the impact of a cholesterol-rich diet on the gallbladder. Material and methods. The study included ten eight-week-old C57BL6 male mice, which were divided into two study groups and fed different diets for 48 h: a hypercholesterolemic diet and a balanced Chow diet. After 48 h, the mice were analyzed by US with a Siemens Acuson Antares equipment. Mice were subsequently sacrificed to carry out a cholesterol analysis with a Refloton System (Roche), a crystal analysis with a Carl Zeiss microscope with polarized light, and a histological analysis with Hematoxylin- eosin staining. Results. The hypercholesterolemic diet induced an increase in gallbladder size and total cholesterol content in the bile, along with important histological changes. Conclusion. Cholesterol overloads not only trigger hepatic damage, but also affect the gallbladder significantly.


REFERENCES

  1. Popkin BM, Adair LS, Ng SW. Global nutrition transition and the pandemic of obesity in developing countries. Nut Rev 2012; 70: 3-21.

  2. Asgharpour A, Vincent R, Min HK. Of mice and men: a review of dietary murine models of nonalcoholic fatty liver disease (NAFLD) and how it correlates to human disease. Austin J Clin Med 2014; 1: 1-5.

  3. Koo SH. Nonalcoholic fatty liver disease: molecular mechanisms for the hepatic steatosis. Clin Mol Hepatol 2013; 19: 210-5.

  4. Kleiner DE, Brunt EM, Van Natta M, Behling C, Contos MJ, Cummings OW, Ferrell LD, et al. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 2006; 41: 1313-21.

  5. Gutiérrez-Ruíz MC, Domínguez-Pérez M, Rodríguez González S, Nuño Lámbarri N, Licona Retama C, Gómez-Quiroz LE. High cholesterol diet modifies the repairing effect of the hepatocyte growth factor. Gac Med Med 2012; 148: 236-42.

  6. Bhatia LS, Curzen NP, Calder PC, Byrne CD. Non-alcoholic fatty liver disease: a new and important cardiovascular risk factor? Eur Heart J 2012; 33: 1190-200.

  7. Vernon G, Baranova A, Younossi ZM. Systematic review: the epidemiology and natural history of non-alcoholic fatty liver disease and non-alcoholic steatohepatitis in adults. Aliment Pharmacol Ther 2011; 34: 274-85.

  8. Chen Y, Kong J, Wu S. Cholesterol gallstone disease: focusing on the role of gallbladder. Lab Invest 2015; 95: 124-31.

  9. Méndez SN, Uribe M. Inhibición de la absorción de cholesterol. Una nueva estrategia para el tratamiento medico de la litiasis de cholesterol. Medicina Universitaria 2008; 10: 230-4.

  10. González-Hita M, Bastidas Ramírez BE, Panduro-Cerda A. Factores de riesgos en la genesis de la litiasis vesicular. Inv Salud 2005; VII: 71-8.

  11. Marí M, Caballero F, Colell A, Morales A, Caballeria J, Fernandez A, Enrich C, et al. Mitochondrial free cholesterol loading sensitizes to TNF- and Fas-mediated steatohepatitis. Cell Metab 2006; 4:185-98.

  12. Abeysuriya V, Deen KI, Navarathne NM. Biliary microlithiasis, sludge, crystals, microcrystallization and usefulness of assessment of nucleation time. Hepatobiliary Pancreat Dis Int 2010; 9: 248-53.

  13. Everhart JE, Khare M, Hill M, Maurer KR. Prevalence and ethnic differences in gallbladder disease in the United States. Gastroenterology 1999; 117: 632-9.

  14. Muna Z, Rana M, Khalida S. Histological changes of gallbladder mucosa: correlation with various types of cholelithiasis. Iraqi J Comm Med 2011; 24: 234-40.

  15. Leopold GR, Amberg J, Gosink BB, Mittelstaedt C. Gray scale ultrasonic cholecystography: a comparision with conventional radiographic techniques. Radiology 1976; 121: 445-8.

  16. Khanuja B, Cheah YC, Hunt M, Nishina PM, Wang DQ, Chen HW, Billheimer JT, et al. Lith 1 a major gene affecting cholesterol gallstone formation among inbred stains of mice. Proc Natl Acad Sci USA 1995; 92: 7729-33.

  17. Wang DQ, Paigen B, Carey MC. Phenotypic characterization of Lith genes that determine susceptibility to cholesterol cholelithiasis in inbred mice: physical-chemistry of gallbladder bile. J Lipid Res 1997; 38: 1395-411.

  18. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación, Norma Oficial Mexicana NOM-062-200- 1999. Especificaciones técnicas para la producción, cuidado y uso de los animals de laboratorio. Diario Official de la Federación 2001; 2: 20-74.

  19. López-Reyes AG, Martínez-Flores K, Clavijo-Cornejo D, Nuño-Lambarri N, Palestino-Dominguez M, Souza V, Bucio L, et al. Cholesterol overload in hepatocytes affects nicotinamide adenine dinucleotide phosphate oxidase (NADPH) ac tivity abrogating hepatocyte growth factor (HGF) induced cellular protection induced cellular protection. Gac Med Mex 2015; 151: 456-64.

  20. Dominguez-Perez M, Nuño-Lambarri N, Clavijo-Cornejo D, Luna-López A, Souza V, Bucio L, Miranda RU, et al. Heptatocyte growth factor reduced free cholesterol-mediated lipotoxicity in primary hepatocytes by countering oxidative stress. Oxid Med Cell Longev 2016; doi:10.1155/2016/ 7960386

  21. López-Islas A, Chagoya-Hazas V, Pérez-Aguilar B, Palestino- Dominguez M, Souza V, Miranda RU, Bucio L, et al. Cholesterol enhances the toxic effect of ethanol and acetaldehyde in primary mouse hepatocytes. Oxid Med Cell Longev 2016; doi: 10. 1155/2016/9209825

  22. Sahlin S, Granstrom L, Gustafsson U, Stahlberg D, Backman L, Einarsson K. Hepatic esterification rate of cholesterol and biliary lipids in human obesity. J Lipid Res 1994; 35: 484-90.

  23. Bennion LJ, Grundy SM. Effects of obesity and caloric intake on biliary lipid metabolism in man. J Clin Invest 1975; 56: 996-1011.

  24. Shaffer EA, Shaffer EA, Small DM. Biliary lipid secretion in cholesterol gallstone disease. The effect of cholecystectomy and obesity. J Clin Invest 1977; 59: 828-40.

  25. Einarsson K, Angelin B. Pathogenesis of cholesterol gallstone disease: the secretory defect. In Bile Acids in Health and Disease. T. Northfield, R. Jazrawi and P. Zentler-Munro, editors. Kluwer Academic Publishers Dordercht; 1998, p. 99-116.

  26. Hussaini SH, Pereira SP, Murphy GM, Dowing RH, Deoxycholic acid influences cholesterol solubilization and microcrystal nucleation time in gallbladder bile. Hepatology 1995; 22: 1735-44.

  27. Treviño-García M, De la Peña Gil V, Reyna Guerra JM, Sánchez Ávila MT, Cano Muñoz I, Guardiola Fernández A, Fernández de la Torre, et al. Tiempo de aparición y frecuencia de patología hepatobiliar en pacientes con nutrición parenteral total. Memorias del 38 Congreso de Investigación y Desarrollo: Ecosistemas para el desarrollo emprendedor, económico y social; 2008, p. 495.

  28. Hanbidge AE, Buckler PM, O’Malley ME, Wilson SR. From the RSNA refresher courses: imaging evaluation for acute pain in the right upper quadrant. Radiographics 2004; 24: 1117-35.

  29. Portincasa P, Van de Meeberg P, Van Erpecum KJ, Palasciano G, Van Berge-Henegouwen GP. An update on the pathogenesis and treatment of cholesterol gallstones. Scand J Gastroenterol Suppl 1997; 223: 60-9.

  30. Gerke H, Baillie J. Biliary microlithiasis a neglected cause of recurrent pancreatitis, and biliary colic? J Gastroenterol 2004; 20: 499-501.

  31. Konikoff FM, Laufer H, Messer G, Gilat T. Monitoring cholesterol crystallization from lithogenic model bile by time-lapse density gradient ultracentrifugation. J Hepatol 1997; 26: 703-10.

  32. Jüngst C, Kulluak-Ublick GA, Ju¨ngst D. Gallstone disease: Microlithiasis and sludge. Best Pract Rest Clin Gastroenterol 2006; 20: 1053-62.

  33. Landi K, Sinard J, Crawford JM, Topazian M. Cholesterol Crystal morphology in acalculous gallbladder disease. J Clin Gastroenterol 2003; 36: 364-6.

  34. Van Erpecum KJ, Wang DQ, Moschetta A, Ferri D, Suelto M, Portincasa P, Hendrickx JJ, et al. Gallbladder histopathology during murine gallstone formation: relation to motility and concentrating function. J Lipid Res 2006; 47: 32-41.

  35. Chang HJ, Such JI, Kwon SY. Gallstone formation and gallbladder mucosal changes in mice fed a lithogenic diet. J Korean Med Sci 1999; 14: 286-92.




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