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ISSN 0016-3813 (Print)

2018, Number 2

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Gac Med Mex 2018; 154 (2)

Citotoxicidad de soluciones recomendadas para el almacenamiento de dientes avulsionados en cultivo con células del ligamento periodontal

Cruz-Durán G, Ortiz-Daza R, Díaz-Acevedo JA, Sánchez-Trocino B, Arenas-Arrocena MC, García-Contreras R

Language: Spanish
References: 18
Page: 217-221
PDF size: 188.76 Kb.


ABSTRACT

Introduction: The medium for avulsed teeth storage until their reimplantation is key to the preservation of human periodontal ligament fibroblasts (HPLF). Objective: Our purpose was to compare the cytotoxic effect of milk and isotonic solution, used for the storage of avulsed teeth, on the preservation of HPLF. Method: A subculture of periodontal ligament fibroblasts was carried out with a density of 1:2 (3 ×105 cells/mL) and was incubated for 48 hours. The cells were divided in two groups, which were placed either in milk or isotonic solution for 24 hours at 5% CO2, 37 ºC and 95% humidity. The number of viable cells was determined with a colorimetric fast assay by the reduction of MTT and mitochondrial activity. Data were processed with the Shapiro‑Wilk normality test, Student’s t‑test and paired Student’s t‑test (with significance set at 0.05). Results: The cells exposed to milk for 24 hours showed statistically significant cytotoxicity at concentrations of 0.09, 0.39, 0.78, 1.56, 3.125, 6.25 and 50%. HPLFs exposed to isotonic solution showed no significant reduction in the number of cells at concentrations of 25 and 50%. Conclusion: Isotonic solution appears to be better for HPLF 24‑hour storage in comparison with whole milk.


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