Bisset LJA, Rodríguez CMM, Piedra OLA, Rey J, Marquetti FMC, Gutiérrez BG

Language: Spanish
References: 0
Page: 1-10
PDF size: 120.91 Kb.

ABSTRACT

Objective: determine the susceptibility of Aedes aegypti (L.) mosquitoes to three insecticide formulations using the impregnated bottle bioassay, standardized by the Centers for Disease Control and Prevention (CDC). Methods: an evaluation was conducted of A. aegypti field populations collected in Havana and Pinar del Río in relation to three of the insecticides most commonly used for A. aegypti control in Cuba: Galgotrin 25 EC (cypermethrin), Aqua k-othrine 2 EW (deltamethrin) and Clorcide 44 EC (chlorpyrifos) using the CDC impregnated bottle method. Analysis was also performed of three insecticide resistant reference strains and the Rockefeller susceptible reference strain. Determination was made of the time when the bottles may be effectively used after impregnation with each insecticide. Results: most of the field populations evaluated were found to be resistant to Aqua k-othrine 2 EW and Clorcide 44 EC, and all of them showed susceptibility to Galgotrin 25 EC. The reference A. aegypti strains resistant to temephos, deltamethrin and propoxur showed high resistance to the study formulations, whereas the Rockefeller strain showed susceptibility. The bottles may be used effectively 4-15 days after impregnation, and be reused up to 5 times, depending on the storage time. Conclusions: the CDC bottle bioassay may be a useful tool to evaluate the susceptibility of insecticide formulations in Cuban A. aegypti field populations. Galgotrin 25 EC is still effective for A. aegypti control in Cuba.