González BM, Martínez BLA, Salgado SG

Language: Spanish
References: 12
Page: 960-970
PDF size: 65.61 Kb.

ABSTRACT

Introduction: Thick film is a routine technique consisting of a concentrated blood sample where the erythrocytes are hemoglobinized during the precoloration and coloration that facilitates the identification of the parasites that could be present in the interior. Objective: training with the purpose of training technicians and graduates in clinical laboratory, to make a correct take and reading of the sample in the veracity detection of malaria cases. Method: the training was directed to all Cuban collaborators licensed or clinical laboratory technicians from Venezuela, to complement in a theoretical-practical way how the procedure is performed to take, read and quantify the thick films, issue a correct laboratory diagnosis and to raise the quality of the service provided by the Cuban Medical Mission in the State of Bolivar and throughout the country. Results: all Cuban technical collaborators or graduates (100 %) received the training. Conclusion: knowledge and skills were developed in laboratory technicians and graduates in clinical bioanalysis to adequately perform the collection, reading, staining and quantification of the thick films for the diagnosis of malaria. The trainees the fundamental characteristics of malaria as an endemic disease recognized it. The morphological characteristics of the elements were identified with emphasis on the erythrocyte and morphological alterations of the parasitized erythrocytes. The trainees were able to master the techniques of sampling, reading, coloring and quantification of thick films.

REFERENCES

1. Venezuela. Escuela de Malariología. Guías prácticas del Curso Internacional de Malaria y Saneamiento Ambiental. Maracay: Ministerio de Salud y Desarrollo Social; 2012.

2. Venezuela. Dirección General de Salud Ambiental. Manual para la capacitación en el diagnóstico parasicológico de malaria. Maracay: Ministerio del Poder Popular para la Salud; 2013.

3. Costa Rica. Ministerio de Salud. Malaria. San José: MinSal; 2006.

4. Organización Panamericana de la Salud. Diagnóstico de Malaria. Washington D.C.: OPS; 1988. (Publicación Científica No. 512).

5. Mendoza Ruiz LM. La sangre. [Internet]. 2006 [citado 06 Jun 2016]. Disponible en: http://www.monografias.com/trabajos/sangre/sangre.shtml.

6. Perú. Instituto Nacional de Salud. Manual de Procedimientos de laboratorio para el diagnóstico de malaria [Internet]. Lima: INS; 2003. (Serie de Normas Técnicas Nº 39). Disponible en: http://www.ins.gob.pe/repositorioaps/0/4/jer/-1/Antimalaricos/manualMALARIA.pdf.

7. Ng OT, Ooi EE, Lee CC, Lee PJ, NG LC, Pei SW, et al. Naturally acquired human Plasmodium knowlesi infection, Singapore. Emerg Infect Dis [Internet]. 2008 [citado 06 Jun 2016]; 14(5):814-6. Disponible en: https://wwwnc.cdc.gov/eid/article/14/5/07-0863_article.

8. Descripción general de la sangre y sus componentes [Internet]. 2015 [citado 05 Jun 2016]. Disponible en: http://healthlibrary.uchospitals.edu/Spanish/DiseasesConditions/Pediatric/Blood/90,P05425.

9. Center for Diseases Control and Prevention. Malaria [Internet]. [citado 06 Jun 2016]. Disponible en: https://www.cdc.gov/malaria/.

10. Adebami OJ, Owa JA, Oyedeji GA, Oyelami OA, Omoniyi Esan GO. Associations between placental and cord blood malaria infection and fetal malnutrition in an area of malaria holoendemicity. Am J Trop Med Hyg [Internet]. 2007 [citado 06 Jun 2016]; 77(2): 209-13. Disponible en: http://www.ajtmh.org/docserver/fulltext/14761645/77/2/0770209.pdf?expires=1508944300&id=id&accname=guest&checksum=E45EE97D285439896B3D806910860F6F.

11. Benito Llanes A, Roche Royo J, Rubio Muñoz JM, Alonso Fernández PL. Enfermedades infecciosas (VII). Paludismo. Medicine. 1998; 7(81): 3753-9.

12. Biagini GA, Pasini EM, Hughes R, De Koning HP, Vial HJ, O'Neill PM, et al. Characterization of the choline carrier of Plasmodium falciparum: a route for the selective delivery of novel antimalarial drugs. Blood [Internet]. 2004 [citado 06 Jun 2016]; 104(10):3372-7. Disponible en: http://www.bloodjournal.org/content/bloodjournal/104/10/3372.full.pdf?sso-checked=true.