Zambrano MP, Bustamante PKE

Language: Spanish
References: 17
Page: 1-8
PDF size: 123.88 Kb.

ABSTRACT

Introduction: Justicia secunda Vahl. leaves are widely used by rural populations from the Ecuadorian coast for medicinal purposes, but the properties attributed to it have not been verified. A study was conducted of hydroalcoholic, alcoholic and etheric leaf extracts.
Objectives: Characterize the crude drug and several extracts of J. secunda to determine the presence of metabolites or active principles with medicinal potential, and analyze their possible usefulness to produce pharmaceutical products.
Methods: An analysis was conducted of the physicochemical parameters of the crude drug. The leaves were collected at night and dried to constant weight in a stove at 30 °C ± 1 °C for 5 days. They were then crushed in a mortar and weighed. The extracts were obtained by maceration in various organic solvents, such as 90% ethanol, diethyl ether and a 50 % v/v hydroalcoholic solution. For phytochemical analysis, qualitative techniques were applied to determine the main metabolites present in the extracts. Gravimetric techniques were used for determination of total ashes, total solids and percentage of humidity.
Results: It was found that content of total ashes and residual humidity in the crude drug are within the limits established by USP 37 for items of botanical origin. Additionally, alkaloids, coumarins and phenolic glucosides were found to be present in the hydroalcoholic extract; tannins, phenolic glucosides and triterpenes and/or steroids in the alcoholic extract; and alkaloids and triterpenes and/or steroids in the etheric extract.
Conclusion: The great variety of chemical components present in the leaves of J. secunda grants them high potential for medicinal use as antiseptic, antiinflammatory and antioxidant, among others.

REFERENCES

1. García Lahera JP, León Ávila YM. Notas sobre la presencia de Justicia secunda (Acanthaceae) en Cuba. Revista Infociencia. 2013;17(2).

2. Gomez J, Reyes R, Aguilar I. Chemistry and Pharmacology of Selected Asian and American Medicinal Species of Justicia. En Bioactive Phytochemicals: Perspectives for Modern Medicine. Edición 1. Capítulo 14. Argentina: Dava Publishing House. 1996;457-75.

3. Lans C, Harper T, Georges K, Bridgewater E. Medicinal and ethnoveterinary remedies of hunters in Trinidad. BMC Complement Altern Med. 2001;1:10.

4. Lans C. Ethnomedicines used in Trinidad and Tobago for reproductive problems. J Ethnobiol Ethnomed. 2007;3(13).

5. OMS. Quality control methods for herbal materials. Updated edition of Quality control methods for medicinal plant materials. 1998.

6. Barrese Pérez Y, Hernández Jiménez M. Tamizaje fitoquímico de la droga cruda y extracto fluido de la Guacamaya francesa. Rev Cubana Plant Med. 2002;7(3):2.

7. The United States Pharmacopeial Convention. Farmacopea de los Estados Unidos 37 NF 32. Volumen 1. Estados Unidos de América. 2014.

8. Rojas J, Ochoa V, Ocampo S, Muñoz J. La detección de la actividad antimicrobiana de diez plantas medicinales utilizadas en la medicina folclórica de Colombia: Una posible alternativa en el tratamiento de infecciones no nosocomiales. BioMed Central. 2006;6:2-2.

9. Mpiana P, Ngbolua K, Mudogo V, Tshibangu D, Atibu E, Mbala B et al. The Potential Effectiveness of Medicinal Plants used for the Treatment of Sickle Cell Disease in the Democratic Republic of Congo Folk Medicine: A Review. Progress in Traditional and Folk Herbal Medicine. 2012;1:1-11.

10. Koffi E, Le Guernevé C, Lozano P, Meudec E, Adjé F, Bekro Yet al. Polyphenol extraction and characterization of Justicia secunda Vahl. leaves for traditional medicinal uses. Ind Crops Prod. 2013;49:682-89.

11. Sohounhloue D, Akibou L, Agbangnan l, Bossou A, Koudoro Y, Noudogbessi A et al. Chemical characterization and biological activities of extracts of three plants used in traditional medicine in benin: Tectona grandis, Uvaria chameae and Justicia secunda. AJPCR. 2014;7(5).

12. Correa G, Alcantara A. Chemical constituents and biological activities of species of Justicia. Brazilian Journal of Pharmacognosy. 2011;22(1):220-38.

13. Herrera-Mata H, Rosas-Romero A, Crescente O. Biological activity of "sanguinaria" (Justicia secunda) extracts. Taylor & Francis. 2002;40:206-12.

14. Pérez O, Pinto E. Análisis preliminar del efecto antimicrobiano del extracto etanólico de hojas de chinchamuchina (Justicia secunda) sobre el crecimiento de Escherichia coli y Bacillus cereus. Tesis presentada en opción al título de Licenciado en Tecnología de los Alimentos, Núcleo-Monagas, Universidad de Oriente, Venezuela. 2010.

15. Moswa J, Kapanda N, Mungende D, Okitolonda W, Mayangi M, Mihigo S. et al. Plants as an important source of iron for the treatment of anaemia: case of Justicia secunda.11th NAPRECA Symposium Book of Proceedings Antananarivo, Madagascar. 2003;132-35.

16. Mpiana T, Ngbolua K, Bokota M, Kasonga K, Atibu E, Tshibangu Tet al. In vitro effects of anthocyanin extract from Justicia secunda Vahl. on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. J Blood Transfus. 2010;8(4):248-54.

17. Cantillo J, Baldiris R, Jaramillo B. Evaluación de la toxicidad aguda (CL50) frente a Artemia franciscana y la actividad hemolitica de los extractos acuosos, en diclorometano y metanólico parcial de Justicia secunda (Vahl.) Scientia Et Technica. 2007;1(33).