2004, Number 5
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Med Cutan Iber Lat Am 2004; 32 (5)
Detection of Human Papillomavirus in Basal Cell Carcinoma by Polymerase Chain Reaction
Camargo VBAL, Antunes OMS, Goulart FLR, Rocha A, Machado SAC
Language: English
References: 23
Page: 205-209
PDF size: 129.32 Kb.
Text Extraction
The aim of the present study was to determine the presence of human papillomavirus (HPV) in tumors and sun-nonexposed normal skin from patients with basal cell carcinoma (BCC). Twenty-three patients with BCC had their tumors and axillary skin assayed for the presence of HPV by polymerase chain reaction (PCR), using degenerate primers of the L1 region. Controls were preliminarily established by using viral warts as positive control, and control axillary skin from healthy subjects as negative control. HPV was detected in 60.9% of the tumors and 43.5% of the patient’s axillary skin. This difference was not statistically significant (p › 0.05).
REFERENCES
Shamanin V, Zur Hausen H, Lavergne D, et al. Human papillomavirus infections in nonmelanoma skin cancers from renal transplant recipients and nonimmunosupressed patients. J Natl Cancer Inst 1996; 88: 802-11.
Kaltreider SA, Callahan C. Pathogenesis of malignant eyelid tumors Ophthalmol Clin North Am 2000; 13: 557-69.
Schell BJ, Rosen T, Rady P, et al. Verrucous carcinoma of the foot associated with human papillomavirus type 16. J Am Acad Dermatol 2001; 41: 49-51.
Berkhout RJ. Persistence of human papillomavirus DNA in benign and (pre)malignat skin lesions from renal transplant recipients. J Clin Microbiol 2000; 38: 2087-96.
Gibson GE, Ahmed I. Perianal and genital basal cell carcinomas: A clinicopathologic review of 51 cases. J Am Acad Dermatol 2001; 45: 45-68.
Harwood CA, Surentheran T, McGregor JM, et al. Human papillomavirus infection and nom-melanoma skin cancer in immunosuppressed and immunocompetent individuals. J Med Virol 2000; 61: 289-97.
Boxman IL, Russel A, Mulder LH, et al. Case-control study in a subtropical australian population to assess the relation between non-melanoma skin cancer and epidermodysplasia verruciformis human papillomavirus DNA in plucket eyebrow hairs. The Nambour Skin Cancer Prevention Study Group. Int J Cancer 2000; 86: 118-21.
Rust A, McGovern RM, Gostout BS, et al. Human papillomavirus in cutaneous squamous and cervix of a patient with psoriasis and extensive ultraviolet radiation exposure. J Am Acad Dermatol 2001; 44:681-6.
Mullis K, Faloona F, Scharf S, et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Sring Harbor Symp Quant Biol 1986; 51: 263-73.
Strand A, Rylander E. Human papillomavirus: Subclinical and atypical manifestations. Dermatol Clin 1998; 16: 817-27.
Eliezri YD, Silverstein SJ, Novo GJ. Ocurrence of human papillomavirus type 16 DNA in cutaneous squamous and basal cell neoplasms. J Am Acad Dermatol 1990; 23:836-42.
Pierceall WE, Goldberg LH, Ananthaswamy HN. Presence of human virus type 16 DNA sequences in human nonmelanoma skin cancers. J Invest Dermatol 1991; 97:880-4.
Saegusa M, Takano Y, Hashimura M. HPV type 16 in conjunctival and junctional papilloma, dysplasia, and squamous cell carcinoma. J Clin Pathol 1995; 48: 1106-10.
Boyd AS, Annarella M, Rapini RP, et al. False-positive polymerase reaction resuklts for human papillomavirus in lichen planus: potential laboratory pitfalls of this procedure. J Am Acad Dermatol 1996; 35: 42-6.
De Villiers E, Lavergne D, McLaren, et al. Prevailing papillomavirus types in non-melanoma carcinomas of the skinin renal allograft recipients. Int J Cancer 1997; 73: 356-61.
Astori G, Lavergne D, Benton C, et al. Human papillomaviruses are commonly found in normal skin of immunocompetent hosts. J Invest Dermatol 1998; 110: 752-5.
Shamanin V, Glover M, Rausch C, Proby C, et al. Specific types of human papillomavirus found in benign proliferations and carcinomas of the skin in immunosupressed patients. Cancer Research 1994; 54: 4610-3.
Stark LA, Arends MJ, McLaren KM, et al. Prevalence of human papillomavirus DNA in cutaneous neoplasms from renal allograft recipients supports a possibile viral role in tumor promotion. Br J Cancer 1994; 69:222-9.
Ting Y, Manos M. Detection and typing of genital human papillomaviruses. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ - PCR Protocols: a guide to methods and applications. San Diego: Academid Press, 1990: 356-67.
Melski JW, Tanenbaum L, Parrish JA, et al. Oral methoxsalen photochemotherapy for the treatment of psoriasis: a cooperative clinical trial. J Invest Dermatol 1977; 68: 328-35.
Ito Y, Evans Ca. Induction of tumors in domestic rabbits with nucleic acid preparations from partially purified Shop papilloma virus and from extracts of the papillomas of domestic and cottontail rabbits. J Esp Med 1961; 114: 485-91.
Moy RL, Eliezri YD. Significance of human papillomavirus-induced squamous cell carcinoma to dermatologists. Arch Dermatol 1994; 130: 235-8. (Editorial).
McDougall JK. Cofators in the progression of HPV-associated tumors. Antibiot Chemother 1994; 46: 150-64.