Rodrigues CA, Silvino PP, Marivando BL, Duarte AE, Vega GMC, Rolón M, Soares VCA, Josicleide MA, Bezerra MBMF, Coutinho HD

Language: Portugués
References: 47
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ABSTRACT

Introduction: Leishmaniasis is a complex of tropical diseases caused by more than twenty different species of intracellular protozoa from the genus Leishmania. Chagas disease is one of the main public health problems in many countries. It mostly affects the poor, rural population. Melissa officinalis L. is a perennial herb, an exotic plant from the family Lamiaceae which has been widely used in folk medicine for therapeutic purposes. Its essential oil has been used as antioxidant, antifungal, antiviral and sedative.
Objective: Evaluate the cytotoxicity, chemical composition and antiparasitic activity of essential oil of the species M. officinalis.
Methods: M. officinalis specimens were collected from the municipality of Crato, CE, Brazil, and deposited in the herbarium at the Regional University of Cariri (URCA). Analysis of the chemical composition of essential oil of M. officinalis was performed by gas chromatography / mass spectrometry. L. braziliensis and Trypanosoma cruzi promastigotes were used for the study of in vitro leishmanicidal activity. Mammalian fibroblast strain NCTC clone 929 was used to evaluate cytotoxic activity.
Results: Analysis of essential oil from M. officinalis by GC / MS led to identification of a total 12 constituents 99.83%, among which some were a majority: geranial, Z citral, limonene and 1-carvone. Toxicity against fibroblasts was found to be low. M. officinalis displayed greater capacity to avoid replication against the promastigote form of the parasite at the concentrations assayed.
Conclusion: The species may be an important source of new agents and selective media for the treatment of tropical diseases caused by protozoa, whose properties should be studied with respect to their mechanisms.

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