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ISSN 1561-3011 (Electronic)

2017, Number 1

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Rev Cubana Invest Bioméd 2017; 36 (1)

Polystyrene latex synthesis and its application in the serotyping of Neisseria meningitidis serogroup W

Marrero CG, Ramírez BF, Pérez DL, Musacchio LA, González AE, Otero AO, Acevedo GR, Bada RN, Amey RY, Zaldívar SD, Menéndez MT

Language: Spanish
References: 17
Page: 1-12
PDF size: 269.33 Kb.


ABSTRACT

Introduction: Polystyrene spheres are used as solid support for biomolecules in latex immunoagglutination assays performed to diagnose disease and identify microorganisms.
Objectives: Synthesize and characterize three consecutive polystyrene latex lots and their application in developing reagents for the typing of the bacteria Neisseria meningitidis serogroup W.
Methods: Synthesis was based on polymerization in surfactant-free emulsion. Morphology and cleanliness of the particles was assessed by scanning electron microscopy. Diameter and Z-potential of the spheres was determined by dynamic dispersion and light electrophoresis. Fourier transform infrared (FT-IR) spectroscopy was used as identity assay. Study of the capacity of spheres to adsorb proteins was based on monoclonal antibody (mAb) 5C11F1, specific for the capsular polysaccharide of N. meningitidis serogroup W.
Results: Three consecutive polysterene latex lots were obtained, with spheres of a mean diameter in the 0.8 µ 0.1 µm interval, showing their similarity with three commercial latexes in terms of cleanliness, morphology and FT-IR spectra. The latexes were stable for at least 24 months, coinciding with the Z-potentials measured below -30 mV. Particle surfaces were saturated in the interval of 48 to 63 µg of mAb per mg of spheres for the three latex lots and for a commercial latex used as control. The latex reagents obtained by adsorption of mAb 5C11F1 to polystyrene spheres were successfully applied in the identification of strains of N. meningitidis serogroup W.


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