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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2005, Number 1

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Rev Biomed 2005; 16 (1)

Molecular diagnosis of a bronchiolitis outbreak in Cienfuegos, Cuba 2002.

Savón-Valdés C, Valdés-Ramírez O, González-Muñoz G, Palerm-Caravallo L, González-Báez G, Goyenchea-Hernández A

Language: Spanish
References: 37
Page: 13-19
PDF size: 62.81 Kb.


ABSTRACT

Introduction. Bronchiolitis is an acute disease of the respiratory airway which appears during infancy; its clinical features begin with a common cold. But in more than a half of these patients it progresses to a lower respiratory tract infection requiring hospitalization mainly in the population under 1 year of age. The main agents which are able to produce this clinical feature are the Human Respiratory Syncytial virus (HRSV) followed by the Parainfluenza virus type 3 (HPiV-3). The isolation of both viruses is difficult, so sensitive molecular techniques were applied. The main objective of this study was to determine the etiological agents of this bronquiolitis outbreak in infants under 6 months of age in Cienfuegos Province during 2002.
Materials and Methods. A total of 19 clinical samples (nasopharyngeal swabs) were collected between June and July 2002 from infants admitted to the respiratory ward of the Paediatric University Hospital Paquito González Cueto in Cienfuegos with a clinical diagnosis of bronchiolitis. To confirm the clinical diagnosis two Multiplex PCR were applied; the first one for the diagnosis of HRSV A and B and the second for the simultaneous detection of HPiV 1, 2, and 3.
Results. Of the 19 samples studied 8 were positive, and 42% of general positivity was obtained. 3 of (16%) were positive to HVSR Subtype A and 5 (26.3%) to HPIV-3, including the fatal case.
Conclusions. The application of the molecular diagnosis using these multiplex PCR, allowed us to give a rapid and accurate answer to the Health authorities about the etiology of this outbreak in a high risk age group which is the population under 1 year of age.


REFERENCES

  1. 1.- Welliver J R, Welliver RC. Bronchiolitis. Pediatr Rev 1993;14:134-9.

  3. 2.- Neves JL, Fonseca C, Cardoso MJ, Azevedo A, Bonito A. Relación entre subtipo del Virus Respiratorio Sincitial y la gravedad Clínica en la bronquiolitis. An Esp Ped 2001; 54 (6): 558-65.

  5. 3.- Hall BC. Respiratory syncytial virus and parainfluenza virus. N Engl J Med 2001; 344:1917-28.

  7. 4.- Cane PA. Molecular epidemiology of respiratory syncytial virus. Rev Med Virol 2001; 11:103-16.

  9. 5.- Freymuth F, Vabret F, Galateau-Salle F, Ferey G, Eugene J, Petitjean E, et al. Detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequence in respiratory tract of infants by polymerase chain reaction and hybrydisation.Clin Diag Virol 1997; 8: 31-40.

  11. 6.- Savón C, Valdés O, Aguilar J, Goyenechea A, González-Muñoz G, Palerm L, et al. Diagnóstico del virus sincitial respiratorio en una población pediátrica en dos temporadas epidémicas. (2000-2001).Cuba. Rev Lati Microbiol 2002; 44(Suppl 1):21-2.

  13. 7.- Cancio R, Savón C, Oropesa S, Abreu I, Pérez L, Hernández B et al. Diagnóstico rápido de los principales virus respiratorios en la Ciudad de la Habana 1995-1997. Rev Argentina Microbiol. 2000; 32:21-6.

  15. 8.- Goyenechea A, Bello M, Clua A, Savón C, Valdivia A, Oropesa S, et al. Determinación de los anticuerpos fijadores del complemento al virus sincitial respiratorio. Estudio longitudinal en una población menor de un año. Ciudad de la Habana. Rev Med Trop 1994; 46:79-85.

  17. 9.- Organización Panamericana de la Salud. Manual de Procedimientos Diagnósticos de las Infecciones Respiratorias Agudas de Etiología Viral. La Habana 2003.

  19. 10.- Casas I, Powell L, Klapper P E, Cleator G M. New method for extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay. J Virol Methods 1995; 53:25-36.

  21. 11.- American Type Culture Collection (ATCC).Catalogue of Cell Lines and Hybridomas 7th ed Rockville MD. ATCC; 1992. p47.

  23. 12.- Pérez-Breña P. Estudio comparativo de cepas de virus respiratorio sincitial seleccionadas según criterios clínicos epidemiológicos (disertación de tesis doctoral Universidad Complutense de Madrid 1998).

  25. 13.- Echevarría JE, Erdman D, Swierkoz E M, Holloway B, Anderson L. Simultaneous detection and identification of human Parainfluenza Viruses 1,2 and 3 from clinical samples by Multiplex PCR. J Clin Microbiol 1998; 36:1388-91.

  27. 15.- Valdés O, Martínez I, Valdivia A, Cancio R, Savón C, Goyenechea A, et al. Unusual antigenic and genetic characteristics of human respiratory syncytial virus isolated in Cuba. J Virol 1998; 72:7589-92.

  29. 16.- Hall CB, McCarthy CA. Respiratory Syncytial Virus. En: Mandell Gl, Bennett JE, Dolin R eds. Philadelphia: Churchill Livingstone; 2000. p. 1782-1801.

  31. 17.- Shay DK, Holman RC, Newman RD, Lui LL, Stout J W, Anderson LL. Bronchiolitis associated to hospitalizations among US Children.1980-1996. JAMA 1999; 282: 1440-6.

  33. 18.- Grubor B, Gallup JM , Meyerholz DK, Crouch, EC, Evans RB, Brogden KA et al. Enhanced surfactant protein and defensin mRNA levels and reduced viral replication during Parainfluenza Virus Type 3 peumonia in neonatal lambs. Clin Diagn Lab Immunol 2004; 11:599-607.

  35. 18.- Van Den Hoogen, de Jong JC, Groen J, Kuriken T, Fouchier R, Osterhaus A. Newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nature Medicine 2001; 7:719-24.

  37. 19.- Greensill J, Mcnamara P, Dove W, Flanagan B, SmythR L, Hart A. Human Metapneumovirus in severe respiratory syncytial virus bronchiolitis. Emerg Infect Dis 2003; 18:372-5.




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