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ISSN 0210-5187 (Electronic)

2017, Number 2

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Med Cutan Iber Lat Am 2017; 45 (2)

Desmoglein1, CD44, Syndecan-1, Galectin-3 and EGFR in the normal and hyperplasic epidermis

Carrillo LM, Arciniegas CE, Rojas H, Ramírez R, Reyes O, Ortiz W

Language: Spanish
References: 42
Page: 119-130
PDF size: 1182.03 Kb.


ABSTRACT

Introduction: The participation of some molecules in the architecture and functioning of the skin barrier has not been yet completely studied. Among these molecules are desmoglein1, CD44, syndecan-1, and EGFR which are characterized by exhibiting an N-terminal that possesses lateral chains of glycans, and the galectin-3, a receptor of glycans that contain β-galactoside residues. Objective: To examine the distribution and localization of desmoglein1, CD44, syndecan-1, EGFR and galectin-3 in the normal and hyperplasic epidermis. Material and methods: Biopsies of patients diagnosed with lichen planus, psoriasis vulgaris, and BCC (five per each) were selected. Six samples of normal human skin were also examined. The distribution and localization of desmoglein1, CD44, syndecan-1, EGFR and galectin-3 was examined by immunofluorescence staining using confocal laser scanning microscopy. Results: In the normal epidermis desmoglein1, CD44, syndecan-1, and galectin-3 were detected in the upper most cell layers delineating the cellular margins and intercellular bridges between adjacent keratinocytes with an expression gradient that increased from the basal layer until suprabasal layers, while in the hyperplasic epidermis they were restricted to suprabasal layers, appearing interrupted in the intercellular bridges of many keratinocytes. Conclusions: Our observations suggest that Dsg1, CD44, syndecan-1 and EGFR through their N- and O-glycans would establish associations facilitated by galectin-3 forming a molecular lattice that would contribute to the assembly and stability of desmosomes in the upper most cell layers of the epidermis and that perturbation of this lattice would lead to epidermal hyperplasia.


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