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ISSN 0864-4551 (Print)

2016, Number 4

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Biotecnol Apl 2016; 33 (4)

Changes in peptide and protein concentrations during the ontogenesis of honeybee (A. mellifera) drone larvae is associated to variations in protease activity

Grishina Z, Gengin M

Language: English
References: 23
Page: 4221-4224
PDF size: 444.81 Kb.


ABSTRACT

The development of a bee drone larvae comprises a period of seven days, in which the larva mass increases 1500-fold. There is no comparable increase during the development of any other species, the in metabolic activity is not observed in any other species development. Rapid development of larvae of insects is mediated by key regulators such as regulatory peptides or protease activity acting on those peptides. Therefore, we studied the dynamics of peptide turn-over during larvae ontogenesis by purifying and measuring the peptide amount in larvae, depending on their age, using size-exclusion chromatography and spectrophotometric measurement. Additionally, we measured the activity of proteolytic enzymes such as a cathepsin D and the trypsin-like proteases, by measuring the conversion rate of enzyme specific substrates, and the effect of pH on the enzyme activity was studied using specific substrates, to corroborate the differences to be found in the studied proteolytic enzymes. We demonstrated correlation between the activity of trypsin-like proteases and peptide amount during larvae ontogenesis. This could indicate that these enzymes participate in the production and cleavage of regulatory peptides during larvae ontogenesis, whereas no correlation was observed for cathepsin D activity and peptide amount.


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