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2017, Number 4

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Rev Med MD 2017; 8.9 (4)

Damage to DNA in newborns of overweight mothers

Pérez-Díaz CI, Zamora-Pérez AL, Ortiz-García YM, Sánchez- Urbina R, Avilés-Martínez KI, Pérez- Rulfo ID

Language: Spanish
References: 17
Page: 140-145
PDF size: 672.89 Kb.


ABSTRACT

Introduction. Overweight in pregnancy is a chronic inflammatory process that increases the production of free radicals and oxidative stress. The increase of free radicals can cause ruptures to DNA and form micronuclei, their presence and frequency represents genomic damage, so the micronucleus assay in exfoliated cells is a specific biomarker to evaluate the exposure to genotoxic, teratogenic and carcinogenic compounds. Therefore, the objective of the present study was to determine the number of micronuclei in oral mucosa cells of mothers with normal weight, overweight and their newborns.
Material and Methods. This was a cross-sectional study, mothers and their newborns were analyzed according to the mother's BMI. Cheek mucosa cells were sampled and stained with acridine orange for analysis under fluorescence microscopy. The non-parametric Mann Whitney U test of the SPSS statistical package was used to establish differences between the groups.
Results. Overweight mothers and their newborns had a higher number of micronuclei compared to the normal weight group (p ‹0.05).
Discussion. Our results show that there is DNA damage in overweight mothers and their newborns. It may be due to the inflammatory process characteristic of overweight and consequent increase of free radicals, showing that the intrauterine environment may be prone to imbalances between pro and anti oxidants that affect the development of the fetus, evidencing the teratogenic potential of obesity.


REFERENCES

  1. Olaiz G, Rojas R, Barquera S, Shamah T, Aguilar C, Cravioto P, López P, Hernández M, Tapia R, Sepúlveda J. Encuesta Nacional de Salud 2000. Tomo 2. La salud de los adultos. Cuernavaca, Morelos, México. Instituto Nacional de Salud Pública, 2003.

  2. González J, Juárez JS, Rodríguez JL. Obesidad y embarazo. Revista Médica MD.2013; 4(4):269-275pp

  3. Ortega LL, López J, Sánchez A, Magaña G, Guzmán A, Angulo E, Gutiérrez J. Evaluación clínica comparativa entre recién nacidos de madres obesas y eutróficas. Pediatría de México. 2012; 14 (1): 12-16.

  4. Blancas G, Almanza JC, López RI, Alarcón FJ, García R, Cruz M. La obesidad como un proceso inflamatorio. Bol. Med. Hosp. Infant. 2010; 67(2): 88-97pp.

  5. Sies H. Biochemistry of oxidative stress. Angewandte Chem 1986; 25:1058-1071.

  6. Heddle JA, Cimino MC, Hayashi M, Romagna F, Shelby MD, Tucker JD, Vanparys P, MacGregor JT. Micronuclei as an index of cytogenetic damage: past, present and future. Environ Mol Mutagen. 1991; 18:277-291pp.

  7. Zúñiga GM, Torres O, Zamora A, Gómez BC, Ramos ML, Gallegos MP, Flores A, López A. Induction of micronucleated erythrocytes in mouse peripheral blood after cutaneous application of 5-Fluorouracil. Arch Med Res. 2003; 34:141-14pp.

  8. Malti N, Merzouk H, Merzouk SA, Loukidi B, Karaouzene N, Malti A, Narce M. Oxidative stress and maternal obesity: feto-placental unit interaction. Placenta 2014; 35(6):411-6pp. doi: 10.1016/j..2014.03.010.

  9. Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res 1992; 271:69-77.

  10. Shashikala R, Indira AP, Manjunath GS, Arathi rao K, and Akshatha BK. Role of micronucleus in oral exfoliative cytology. J Pharm Bioallied Sci. 2015 Aug; 7(Suppl 2): 409–413pp. doi: 10.4103/0975-7406.163472

  11. Esposito K, Ciotola M, Giugliano DJ. Oxidative stress in the Metabolic Syndrome. Endocrinol. Invest. 2006; 29: 791–795.

  12. Pihl E, Zilmer K, Kullisaar T, Kairane C., Magi A, Zilmer M. Atherogenic inflammatory and oxidative stress markers in relation to overweight values in male former athletes. Int. J. Obesity. 2006; 30: 141–146pp.

  13. Boveris A. La evolución del concepto de radicales libres en biología y medicina. Ars Pharm. 2005; 46: 85-95.

  14. Hamiyet A, Sahina F, Bayramb F, Bitgena N, Mertc M, Guclud K, Hamurcua Z. Evaluation of chromosomal damage, cytostasis, cytotoxicity,oxidative DNA damage and their association with body-mass indexin obese subjects. Mutation Research. 2014; 771: 30-36pp.

  15. Malti N, Merzouk H, Merzouk S. Oxidative stress and maternal obesity: Feto-placental unit interaction. Placenta. 2014; 35: 411–416 pp.

  16. Gallardo J, Gomez J, Medina P, Juarez F, Contreras A, Galicia M, Sanchez R. Maternal Obesity Increases Oxidative Stress in the Newborn. Obesity. 2015; 23(8).1650- 1654pp.

  17. Vickers M. La programación de desarrollo y la transmisión transgeneracional de la obesidad. Ann Nutr Metab 2014; 64(suppl 1):26–34pp.




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