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ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2016, Number 3

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Rev Mex Periodontol 2016; 7 (3)

Antimicrobial effect of ozone therapy against Streptococcus sanguis as a preventive treatment of periodontal disease: In vitro study

Peralta ER, Vázquez GFJ, Portilla RJ, Carrasco MN, Donohué CA, Cuevas GJC, Tovar CK, Espinosa CLF, Arroyo MMA

Language: Spanish
References: 41
Page: 84-92
PDF size: 402.83 Kb.


ABSTRACT

The periodontal disease is one of the most prevalent oral diseases in worldwide that includes a bacterial etiology. The Streptococcus sanguis (S. sanguis) is a microorganism that has been strongly associated with the development of periodontal disease. Notwithstanding that there are acceptable treatments, it is necessary to an evaluate alternative antimicrobial agents for the treatment of periodontal disease. The objective of this study was to evaluate variations of the ozone administration and its antimicrobial effect against S. sanguis. Tubes with brain-heart infusion broth were exposed to gaseous ozone to obtain aqueous ozone in several concentrations (0.0042-0.0493 mg/mL) in different times (10-900 s) using various accessories for ozone administration (conventional and modified needle respectively). Antimicrobial assay was made using spread microbiologic tests with blood agar plates. Results indicated that aqueous ozone with modified needle inhibited more significantly the S. sanguis growth using low concentrations and shorter times of exposition compared to conventional ozonized technique, indicating a mechanism dose-time dependent. The ozone therapy could be used as an alternative antimicrobial agent for bacterial growth inhibition of S. sanguis.


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