Izquierdo H, Diosdado E, González CMC, Núñez MC, Cabrera JC, Hernández RM, González JL, Hernández MM, Héctor EF, Gómez R, Proenza R, Velásquez M

Language: English
References: 0
Page: 3511-3516
PDF size: 303.04 Kb.

ABSTRACT

In Cuba, biostimulators are being obtained, based on oligogalacturonides (Pectimorf) and brassinosteroids analogs (Biobras-6, Biobras-16 and MH-5) which were effective when applied in a single phase of the micropropagation processes for plant multiplication. However, the question remained if there could be an improvement when they were applied during the entire micropropagation process and if the effect would be sustained once transferring the plants to ex vitro conditions. In this work, the effectiveness of these biostimulators was evaluated as growth regulators, combined with auxins or cytokinins, in organogenesis and somatic embryogenesis of economically relevant crops, assessed thoruhg their biological activity, and the physiology and anatomy of plants. Regenerants showed genetic stability, the compounds been more effective than the auxin-cykokinin combinations. Pectimorf unprecedently influenced on hormones’ concentration in banana plants during their acclimatization phase, and new methodologies were proposed for micropropagation of garlic, banana and plantain, Spathiphylum sp. and Citrus, not inducing genetic variability, and a protocol for acclimatization of Vriesea. Moreover, pineapple plantlets were obtained of improved quality and uniformity, by combining immersion bioreactors with MH-5 supplementation of the culture medium. It was also corroborated in Arabidopsis sp. and sugarcane that Pectimorf and oligogalacturonides regulate vegetal growth. This research granted the 2015 Award of the Cuban National Academy of Sciences.