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2016, Number 4

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Rev Cubana Hematol Inmunol Hemoter 2016; 32 (4)

Renal pretransplant anti-citomegalovirus and anti-Epstein Barr virus antibodies in Cuba

Marcell RL, Morera BLM, Chang MA, Ustariz GC, Bencomo HA

Language: Spanish
References: 22
Page: 494-505
PDF size: 182.64 Kb.


ABSTRACT

Introduction: Solid organ post-transplant viral infections are the main cause of worldwide morbi-mortality in transplanted patients. In Cuba it has been recently introduced the IgM and IgG anti Epstein Barr (EBV) and anti Citomegalovirus (CMV) antibody detection by ELISA with automatic analyzers as part of the pre transplant studies.
Objective: to know population viral infection prevalence and to find possible association between anti EBV and anti CMV antibodies with sensitizing events and anti-HLA antibodies.
Methods: An, investigation was carry out using 1179 samples from patients waiting for renal transplant at the Institute of Hematology and Immunology since August 2013 to December 2014. Four enzyme immunoassay (ELISA) heterogeneous type, non-competitive, quantitative and indirect were performed using commercial kits: Cytomegalovirus IgG ELISA, IgM ELISA Cytomegalovirus, Epstein-Barr virus VCA IgG and Epstein-Barr virus VCA IgM. Alloimmunization state anti-HLA class I and II are defined according to studies by ELISA with commercial kits: LIFECODES QuikScreen and LIFECODES B-Screen. Chi square test of independence was used to determine the existence of association between the presence of antibodies and sex, blood transfusions, previous transplantation, hepatitis B, C and anti-HLA antibodies.
Results: It was found that the viral infection prevalence was the same as other populations, association of IgM anti CMV, IgG anti CMV and IgM anti EBV with sex and IgG anti CMV with blood transfusions, hepatitis C seropositivity and anti-HLA clase I antibodies.
Conclusions: It is necessary to take measures to avoid peritransplant contagion of seronegative patients to theseviruses by blood transmission due to the immunosuppression that they cause, in order to obtain a renal transplant success.


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