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2016, Number 1

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Rev Cubana Farm 2016; 50 (1)

Preliminary evaluation of the antiviral activity of Laurencia obtuse extract against herpesvirus and dengue virus

Rojas PL, Álvarez VM, Morier DLF, Valdés IO, del Barrio AG

Language: Spanish
References: 33
Page: 106-116
PDF size: 170.56 Kb.


ABSTRACT

Introduction: herpes simplex and dengue viruses are the most important human pathogens with high levels of morbidity and mortality. Lack of vaccine development for these viruses, non-existence of drugs for dengue treatment and the emergence of new herpes virus variants resistant to drugs currently in use reinforce the need for new sources of antiviral drugs. Algae remain an interesting alternative in this regard, due to the diversity of compounds with biological activity found in these organisms.
Objective: to evaluate the in vitro antiviral activity of a hydroalcoholic extract of the red seaweed Laurencia obtusa against herpes simplex type 1, herpes simplex type 2 and dengue virus.
Methods: the mean cytotoxic concentration was determined by using the MTT reduction assay in Vero and C6/36HT cells. Mean effective concentration was estimated with the cytopathic effect inhibition in Vero or C6/36HT cells depending on the virus. Selective index (SI) =CC50/EC50 was calculated.
Results: hydroalcoholic extract from L.obtusa was not toxic at the evaluated concentrations The extract managed to inhibit HHV 1 y HHV 2 virus replication in Vero cells with SI values higher than 29 and 42, respectively. On the other hand there was no inhibition of DENV-2 replication in C6/36HT cells.
Conclusions: hydroalcoholic extract from L. obtusa showed in vitro antiviral activity against HHV 1 and HHV 2 and could be employed as a source for new antiviral compounds. This is the first report on the antiviral activity of this alga species.


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