Dora Castro GDA, Diosdado VF, Campomanes CA, Rosales OC, Morilla GA

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ABSTRACT

Attenuated virus vaccines are currently used in most of the countries to control Aujeszky´s disease (AD). There has not been true experience with these vaccines in Mexico, therefore, the objective of this work was to evaluate the immunogenicity and infectivity of the attenuated virus gE-/TK- Begonia strain against AD. The experiment was carried out in a farrow- to finish farm free of AD where all sows (90), and 10% of 4 to 6 month old pigs were bled at the beginning and at the end of this experiment. Three groups of 14 animals each were formed. From each group, seven pigs were vaccinated with two doses of the vaccine; one at 10 and another at 14 weeks of age, and the remaining seven were left as controls to detect viral shedding. Blood samples were taken from all the animals at 10, 14, 18 and 22 weeks of age. Antibody concentration was determined against the whole virus by the ELISA test. Results were that none of the sows or fatteners at the beginning or end of the trial presented antibodies against AD. Mean- and standard deviation of the optical density of the vaccinated animals were: 0.083 ± 0.002 at 10 weeks of age, 0.513 ± 0.025 (P ‹ 0.05) at 14, 0.918 ± 0.068 (P ‹ 0.05) at 18 and 0.907 ± 0.093 (P ‹ 0.05) at 22 weeks of age. None of the sera taken from control animals had optical densities above 0.082 ± 0.02. It was concluded that this attenuated virus vaccine was immunogenic for all the animals, and was not infective for control pigs or other animals in the farm.