Díaz SBR, Jiménez-Estrada M, Auró OA

Language: English/Spanish
References: 0
Page: 189-194
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ABSTRACT

To evaluate the parasiticide effect of “Tepozan” (Buddleja cordata), two compounds were extracted: the first one with hexane (aqueous extract) and the second one with methanol (methanol extract). Afterwards, they were tested against Costia necatrix. Two treatments (24 hours among them) were carried out with four different doses (100 mg/l, 50 mg/l and 10 mg/l for the aqueous extract and 100 mg/l, 50 mg/l and 25 mg/l for the methanol one), and were dissolved in fish tank water (one parasitized fish per tank). Results showed no effect of the aqueous extract and a parasite killer efficacy of the methanol extract to 25mg/l, 50 mg/l and 100 mg/l doses, which killed 83.34%, 97.92% y 100% of the protozoan, respectively. Active compound extractions and identification of the methanol extract with chromatographic plate of silica gel were followed, and three fractions (F1, F2, F3) were obtained. Only the F2 fraction showed efficacy to kill C. necatrix, when tested at 50 mg/l dose killing 81.49% of the protozoan. This fraction is a phenylpropanoid glycoside called verbascoside.