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2016, Number S3

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Rev Med Inst Mex Seguro Soc 2016; 54 (S3)

PCR detection of relevant translocations in pediatric acute lymphoblastic leukemia

Guerra-Castillo FX, Ramos-Cervantes MT, Rosel-Pech C, Jiménez-Hernández E, Bekker-Méndez VC

Language: Spanish
References: 23
Page: 302-308
PDF size: 326.64 Kb.


ABSTRACT

Background: In Mexico, leukemia represents the most common type of cancer in the population under 15 years old with a high incidence rate when compared with developed countries. The etiology of leukemia may be unknown, however different factors are involve such as chromosomal translocations. The aim of this work is to detect the molecular alterations: TEL-AML1, MLL-AF4, BCR-ABL minor and E2A-PBX1 in pediatric patients with acute lymphoblastic leukemia.
Methods: 91 bone marrow samples were collected from pediatric patients with acute lymphoblastic leukemia from january 2012 to march 2013 at the Pediatric Hematology Service, Hospital General “Gaudencio González Garza”. Translocations detected (TEL-AML1, MLL-AF4, BCRABL minor and E2A-PBX1) using real time PCR, SYBR Green (Qiagen, Alameda, CA).
Results: 91 samples were processed, the detected frequencies for each translocation were: TEL-AML1 (7.21%), E2A-PBX1 (5.15%). The MLLAF4 and the BCR-ABL minor translocations were not detected in this study.
Conclusions: The frequencies shown in this study are consistent with the data shown in the literature, where TEL-AML1 is the most common translocation found in pediatric patients. It is of relevance to mention that E2A-PBX1 is found in a high frequency in developing countries when compared with developed countries.


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