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ISSN 0028-3851 (Print)

2008, Number 1

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Rev Neurol Neurocir Psiquiat 2008; 41 (1)

Expression of the synthases of nitric oxide induced for lipopolysaccharide in brain of rates

Sánchez-González DJ, González-López GM, Sosa-Luna CA, Trejo-Bahena NI, Martínez-Martínez CM

Language: Spanish
References: 23
Page: 1-8
PDF size: 220.20 Kb.


ABSTRACT

Introduction. Nitric oxide (NO) is formed from the amino acid L-arginine by a family of enzymes, the NO synthases (NOSs), and plays a role in many physiological functions. NO is synthesized in neurons of the central nervous system, where it acts as a neuromediator with many physiological functions, including the formation of memory, coordination between neuronal activity and blood flow, and modulation of pain. In the peripheral nervous system, NO is now known to be the mediator released by a widespread network of nerves, previously recognized as nonadrenergic and noncholinergic. These nerves mediate some forms of neurogenic vasodilation and regulate certain gastrointestinal, respiratory and genitourinary functions. NOS is a heme-containing enzyme with a sequence similarity to cytochrome P-450 reductase. Several isoforms of NOS are now known to exist, two of which are constitutive; neuronal and endothelial and one of which is inducible by LPS (nNOS, eNOS and iNOS, respectively).
Objective. To explore expression and immunolocalization of NOSs in brains isolated from male Wistar rats by LPS.
Methodology. In our model, we used immunohistochemistry technique.
Results. We found moderate expression of NOSs in basal nuclei, while in rhinencephalo and olfactory tracts it was abundant expression.


REFERENCES

  1. American College of Chest Physicians-Society of Critical Care Medicine Consensus Conference. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Crit Care Med 1992; 20(6): 864-74.

  2. Verweij WR, Bentala H, Huizinga-Van der Vlag A, Loenen-Weemaes A, Meijer DKF, et al. Protection against an escherichia coli-induced sepsis by alkaline phosphatase in mice. Shock 2004; 22(2): 174-9.

  3. Su F, Brands R, Wang Z, Verdant C, Bruhn A, Cai Y, et al. Benefical effects of alkaline phosphatase in septic shock. Crit Care Med 2006; 34(8): 1-8.

  4. Rivers EP, Rady MY, Bilkovski R. Choque. En: Tintinalli JE, Gabor D (eds.). Medicina de Urgencias. US: McGrawHill, p. 263-4.

  5. Alderton WK, Cooper CE, Knowles RG. Nitric oxide synthases: structure, function and inhibition. BS 2001; 357: 593-615.

  6. Hanafy KA, Krumenacker JS, Murad F. NO, nitrotyrosine, and cyclic GMP in signal transduction. Med Sci Monit 2001; 7(4): 801-19.

  7. Lortie MJ, Ishizuka SJ, Schwartz D, Blantz RC. Bioactive products of arginine in sepsis: tissue and plasma composition after LPS and iNOS blockade. AJPCELL 2000; 278: 1191-9.

  8. Sánchez-González DJ, Villanueva-López GC, Sosa-Luna CA, Orjuela- Henry DJ, Ortega-Rangel JA, Martínez-Martínez CM, Herrera- González NE. Óxido nítrico en el sistema nervioso central. Neuronas nitrérgicas. Neurol Neurocir Psiquiat 2004; 37(2): 73-8.

  9. Sánchez-González DJ, Hernández-Pando R, Fernández-Guzmán MP, Valdés-Espinosa RA, Martínez-Martínez CM, León- Contreras JC, et al. Producción de iNOS y nitración de proteínas en la aorta de ratas expuestas a ozono. Rev Sanid Milit Mex 2005; 59(4): 223-40.

  10. Sánchez-González DJ, Moro MA, Hernández-Pando R, Castillo- Henkel C, Mendoza R, Herrera-González N, et al. Peroxynitrite formation in aorta isolated from rats exposed to ozone. Free Radic Biol Med 2004; 36(1): S102.

  11. Villanueva C, Sánchez-González DJ, Moro MA, Hernández- Pando R, Castillo-Henkel C, Mendoza R, Herrera-González N. Immunohistochemistry of nitric oxide synthases in lungs of rats exposed to ozone. Free Radic Biol Med 2004; 36(1): S79.

  12. Villanueva C, Sanchez-Gonzalez DJ, Moro MA, Castillo-Henkel C, Floriano-Sanchez E, Herrera-Gonzalez N. Antioxidant Treatment Prevents Effects of Ozone on Nitric Oxide Synthases Expression in Lung. Free Radic Biol Med 2006; 41(1): S97.

  13. Aguilera P, Chánez-Cárdenas ME, Floriano-Sánchez E, Barrera D, Santamaría A, Sánchez-González DJ, et al. Time-related changes in constitutive and inducible nitric oxide synthases in the rat striatum in a model of Huntington’s disease. Neurotoxicology 2007; 28(6): 1200-7.

  14. Floriano-Sanchez E, Villanueva C, Medina-Campos ON, Rocha D, Sanchez-Gonzalez DJ, Cardenas-Rodriguez N, Pedraza-Chaverri J. Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion, and hypochlorous acid and prevents in vivo ozone-induced tyrosine nitration in lungs. Free Radic Res 2006; 40(5): 523-33.

  15. Sánchez-González DJ, Moro MA, Castillo-Henkel C, Hernández- Pando R, Medina-Santillán R, Larios FJ, et al. Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta. Environ Toxicol Pharmacol 2004; 17: 1-7.

  16. Canul-Andrade LP, Rodríguez-Perales MA, López-Chavira A, Pacheco- Ramírez MA, Sánchez-González DJ. Valoración histológica del daño tisular ocasionado por diferentes medios de corte en piel de conejo. Rev Sanid Milit Mex 2007; 61(3): 162-9.

  17. Aguilera-Hernández P, Chánez-Cárdenas ME, Sánchez-González DJ, Floriano-Sánchez E, Díaz-García M, Barrera D, et al. Regulación de las sintasas de óxido nítrico en el estriado de rata en el modelo de la enfermedad de Huntington inducido por quinolínico. Arch Neurocien Mex 2006; 11: 31.

  18. Pacheco-Ramírez MA, Rodríguez-Perales MA, López-Chavira A, Canul-Andrade LP, Martínez-Martínez CM, Sánchez-González DJ. Expresión de las sintasas de óxido nítrico en tumores glómicos de cabeza y cuello. Rev Sanid Milit Mex 2006; 60(6): 369-78.

  19. Sánchez-González DJ, Trejo-Bahena NI. Biología Celular y Molecular. México: Editorial Alfil; 2006.

  20. Sánchez-González DJ, Trejo-Bahena NI. Histología. Biología Celular y Atlas. México: Editorial Alfil; 2007.

  21. Sonoki T, Nagasaki A, Gotoh T, Takiguchi M, Takeya M, Matsuzaki H. Coinduction of Nitric-oxide Synthase and Arginase I in Cultured Rat Peritoneal Macrophages and Rat Tissues in vivo by Lipopolysaccharide. JBC 1997; 272(6): 3689-93.

  22. Sánchez-González DJ. Expresión de las sintasas de óxido nítrico en pulmones de ratas expuestas a ozono. México: Tesis Doctoral, IPN; 2007.

  23. Orozco-Ibarra M, Medina-Campos ON, Sanchez-Gonzalez DJ, Martínez-Martínez CM, Floriano-Sanchez E, Santamaria A, et al. Evaluation of oxidative stress in d-serine induced nephrotoxicity. Toxicology 2007; 229: 123-35.




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