2000, Number 2
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Vet Mex 2000; 31 (2)
Detection of the detoxifying enzymes B esterases in Culex quinquefasciatus (Say), Boophilus microplus (Canestrini), Amblyomma cajennense (Fabricius) and Blatella germanica (L) with the use of zymograms
Villarino GMA, Wagner GG, Suryakant DW
Language: English/Spanish
References: 21
Page: 107-111
PDF size: 70.31 Kb.
ABSTRACT
Crude extracts of specimens of
Culex quinquefasciatus (Say),
Boophilus microplus (Canestrini),
Amblyomma cajennense (Fabricius) and
Blatella germanica (L) were analyzed for B esterase activity using polyacrylamide gel electrophoresis (PAGE). The crude extract of an organophosphate resistant strain of
B. microplus larvae showed differences when compared to an organophosphate susceptible strain of
B. microplus larvae. An intense band was also shown in
Cx. quinquefasciatus organophosphate resistant strain. One band of different molecular weight was shown for the
A. cajenennse larvae crude extract as well as several bands in
Blatella germanica adult crude extracts. Bovine serum and bovine red blood cells were used as the controls.
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