Herrera UMC, Hernández RY, Rodríguez PI, Guerra PM, Lemus SA

Language: Spanish
References: 15
Page: 671-682
PDF size: 296.76 Kb.

ABSTRACT

Introduction: colon cancer occupies the first place within the neoplasias of the digestive system and the second among all the onco-proliferative processes in both sexes. For the evaluation of vaccines and molecular mechanisms specific to the disease, murine models are frequently used, and there is no animal model standardized for these purposes in this context.
Objective: to standardize a RCC model by implanted cells, stable, reproducible and susceptible to be used in later studies.
Method: young female BALB/c mice were distributed in two control (n = 9) and one (n = 9) groups to which subcutaneous (ectopic) CT 26 cells were inoculated. After 35 days euthanasia was practiced and the morphological characteristics and the presence of spontaneous metastases in tumors were studied.
Results: in the animals of the study group, subcutaneous nodules with the characteristics of a low-grade colon adenocarcinoma were found, which were responsible for the weight gain observed in the study group, which was evidenced when the tumors were removed from the group. The weights of the organs studied were increased by tumor influences, but only spontaneous metastases were found in the lungs.
Conclusions: with this study it was possible to standardize a stable, reproducible and susceptible Colorectal cancer model per implanted cell, which could be used in subsequent studies.

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