2016, Number 1-2
<< Back Next >>
Rev Hosp Jua Mex 2016; 83 (1-2)
Prevalencia de agentes virales en pacientes asociados a infecciones respiratorias en vías bajas
Bravata-Alcántara JC, Cortés-Ortiz IA, Méndez-Velázquez JJ, Jiménez-Barba BE, Chávez-Ocaña S, Sierra-Martínez M
Language: Spanish
References: 26
Page: 5-10
PDF size: 168.40 Kb.
ABSTRACT
Background. Acute respiratory infections of the lower tract (IRBs) are the most common infections in children and adults,
it constituting a major health problem in developing countries. In Mexico IRBs occur up to 25 million cases a year. IRBs
produce high morbidity and mortality and are caused up to 90% by: adenovirus, coronavirus, rhinovirus, influenza,
parainfluenza, respiratory syncytial virus (RSV). Traditional diagnostic methods can take from 48 to 72 h, presenting low
sensitivity and limitations for the isolation of pathogens. With the polymerase chain reaction (PCR) we can make an
effective, rapid and inexpensive diagnosis. This study aimed to assess the prevalence of pathogens isolated from patients
diagnosed with IRBs in the Hospital Juárez de México.
Material and methods. 57 samples from patients with IRBs who
were in internal medicine and pediatrics were used. The samples were obtained from sputum trap. The identification of
the virus was using the commercial detection kit SEEGEN
® RV16 by polymerase chain reaction with reverse transcriptase
(RT-PCR) in real time.
Results. Of the 57 samples, it was found that 31/57 were positive for respiratory viruses, of which
14/31 corresponded to respiratory syncytial virus A, 9/31 to respiratory syncytial virus B and 4/31 to rhinovirus.
Conclusions. The usefulness of knowing viral prevalence rate in these patients with IRBs, is the proper management of
targeted drugs, thus avoiding longer hospital stays and thereby decreasing costs.
REFERENCES
Neizil KM, Maynadrd C, Griffin MR, Heagerty P. Winter respiratory viruses and health care use: a population-based study in the northwest United States. Clin Infect Dis 2003; 37(2): 201-7.
Arnold JC, Singh KK, Spector SA, Sawyer MH. Undiagnosed respiratory viruses in children. Pediatrics 2008; 121(3): e631- e637.
Gonzales R, Malone DC, Maselli JH, Sande MA. Excessive antibiotic use for acute respiratory infections in the United States. Clin Infec Dis 2001; 33(6): 757-62.
Barenfanger J, Drake C, Leon N, Mueller T, Troutt T. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J Clin Microbiol 2000; 38(8): 2824-8.
Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung- Lindell A, Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acas Sci USA 2005; 102(36); 12891-6.
Louie JK, Hacker JK, Gonzales R, Mark J, Maselli JH, Yagi S, et al. Characterization of viral agents causing acute respiratory infection in a San Francisco University Medical Center Clinic during the influenza season. Clin Infect Dis 2005; 41(6): 822-8.
Lozano JM. Infeccion respiratoria aguda en niños. Rev Colomb Neumol 1996; 8(3): 133-45.
Henderson FW, Clyde WA, Collier AM, Denny FW, Senior RJ, Sheaffer CI, et al. The etiologic and epidemiologic spectrum of bronchiolitis in pediatric practice. J Pediatr 1979; 95(2): 183-90.
Van den Hoogen BG, Osterhaus DM, Fouchier RA. Clinical impact and diagnosis of human metapneumovirus infection. Pediatr Infect Dis J 2004; 23(1 Suppl.): S25-S32.
Coiras MT, Aguilar JC, Garcia ML, Casas I, Perez-Brena P. Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays. J Med Virol 2004; 72(3): 484-95.
Brittain-Long R, Nord S, Olofsson S, Westin J, Anderson LM, Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 2008; 41(1): 53-6.
Corzo JRG, Velásquez JN, Rugeles CIG, Rodríguez L, Machuca M, Prieto AT, et al. Prevalencia de virus respiratorios en población menor de 5 años con infección respiratoria aguda en Bucaramanga y las provincias Comunera y de García Rovira, Santander, diciembre del 2012 a diciembre del 2013. Iatreia 2015; 27(4-S): 17.
Galván JM, Rajas O, Aspa J. Revisión sobre las infecciones no bacterianas del aparato respiratorio: neumonías víricas. Archivos de Bronconeumología 2015; 51(11): 590-7.
Asner SA, Rose W, Petrich A, Richardson S, Tran D. Is virus coinfection a predictor of severity in children with viral respiratory infections? Clin Microbiol Infect. Epub 2014.
Clemente I, Mañas MD, Alarcón JM, Monroy C, Sidahi M, Yanes J. Infecciones respiratorias: etiología y patrones de resistencia en el hospital general de Ciudad Real. Rev Esp Quimioter 2012; 25(1): 31-6.
Essa S, Owayed A, Altawalah H, Khadadah M, Behbehani N, Al-Nakib W. Mixed Viral Infections Circulating in Hospitalized Patients with Respiratory Tract Infections in Kuwait. Adv Virol. Epub 2015.
García Pérez C, Alfonso Aguilar P. Estratificación epidemiológica de riesgo. Revista Archivo Médico de Camagüey 2013; 17(6): 762-83.
Hustedt J, Vazquez M. The changing face of pediatric respiratory tract infections: how human metapneumovirus and human bocavirus fit into the overall etiology of respiratory tract infections in young children. Yale J Biol Med 2010; 83(4): 193-200.
Bonzel L, Tenenbaum T, Schroten H, Schildgen O, Schweitzer- Krantz S, Adams O. Frequent detection of viral coinfection in children hospitalized with acute respiratory tract infection using a real-time polymerase chain reaction. Pediatr Infect Dis J 2008; 27(7): 589-94.
Speranza AM, Clary AL, Pereira T, Sapoznicoff L, Schenone N. Estudio multicéntrico de infecciones respiratorias agudas bajas en niños hospitalizados menores de 2 años. Arch Argent Pediatr 2003; 101(5): 365-73.
Lucion MF, Juarez MDV, Viegas M, Castellano V, Romanin VS, Grobaporto M, et al. Virus respiratorio sincicial: Patrón clínico epidemiológico en niños internados en un hospital pediátrico durante los años 2000-2013. Arch Argent Pediatr 2014; 112(5): 397-404.
Martínez-Roig A, Salvadó M, Caballero-Rabasco M, Sánchez- Buenavida A, López-Segura N, Bonet-Alcaina M. Coinfección vírica en las infecciones respiratorias infantiles. Archivos de Bronconeumología 2015; 51(1): 5-9.
Rosete Olvera DP, Archundia Sánchez FJ, Cabello Gutiérrez C, Manjarrez Zavala ME. Patogenia de las infecciones respiratorias por virus. Rev Inst Nal Enf Resp Mex 2002; 15(4): 239-54.
Martínez D. Rinovirus: ¿algo más que un resfrío común? Medwave 2010; 10(05): e4523.
Rohde G, Wiethege A, Borg I, Kauth M, Bauer TT, Gillissen A, et al. Respiratory viruses in exacerbations of chronic obstructive pulmonary disease requiring hospitalisation: a case-control study. Thorax 2003; 58(1): 37-42.
Rabagliati R, Serri M, Montecinos L, Azocar T, Ferrés M. Utilidad de la reacción de polimerasa en cadena en tiempo real en el diagnóstico de infecciones por virus respiratorio sincitial en adultos. Rev Chilena Infectol 2007; 24(6): 441-5.
Full text