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2016, Number 2

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Rev Cubana Plant Med 2016; 21 (2)

Antimicrobial activity of products obtained from Piper species (Piperaceae)

da Silva AH, Vieira RWR, Costa FAF, Nunes LE, Pinto DS, Vasconcelos CJI, de Oliveira CMC, Ramalho CRM

Language: Portugués
References: 29
Page: 168-180
PDF size: 170.65 Kb.


ABSTRACT

Introduction: The species of the genus Piper are widely found in the tropical regions of the globe and have numerous therapeutic indications.
Objective: To evaluate the antimicrobial activity of extracts and fractions of four species of Piper and two flavonoids isolated from Piper montealegreanum and determine the minimum inhibitory concentration of the active products.
Methods: The plant material was collected in different regions of the state of Paraíba and in Belém (Pará) and subjected to extraction with ethanol and partition with solvents in increasing degree of polarity. Flavonoids were isolated through chromatographic techniques. The evaluation of antimicrobial activity was performed by disk diffusion method front the three bacterial species: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and three fungi species: Candida albicans ATCC 76645, Candida tropicalis ATCC 13803 and Candida glabrata ATCC 15545.
Results: Almost half of the products tested were able to inhibit the growth of S. aureus with inhibition zones between 8-16 mm. The crude ethanolic extract and hexane fractions and CHCl3 of P. mollicomum leaves showed antibacterial activity against all the tested strains. The hexane fractions of P. arboreum, P. caldense and P. mollicomum showed antifungal activity, emphasizing the formation of 28 mm inhibition zone for the fraction of P. mollicomum. The two flavonoids isolated from P. montealegreanum showed no antimicrobial activity.
Conclusions: preliminary results suggest that some the products obtained from Piper species are a promising source of compounds with antimicrobial activity.


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